Characterization of the genetic diversity and antimicrobial resistance in Mannheimia haemolytica from feedlot cattle

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dc.contributor.authorKlima, Cassidy L.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2011-08-19T19:22:00Z
dc.date.available2011-08-19T19:22:00Z
dc.date.issued2009
dc.degree.levelMasters
dc.descriptionxi, 116 leaves : ill. ; 29 cmen_US
dc.description.abstractMannheimia haemolytica is an opportunistic pathogen in cattle and the main bacterial agent in bovine respiratory disease. Despite its economic importance, few studies have characterized the genetic diversity of M. haemolytica, particularly from feedlots. Three genotyping techniques (BOX-PCR, (GTG)5-PCR and PFGE) were compared to discriminate M. haemolytica and strains from the family Pasteurellaceae. PFGE was the most discriminating and repeatable, although BOX-PCR was most accurate in clustering isolates together according to species. Mannheimia haemolytica was isolated from nasal swab samples collected from cattle upon entry and exit from two feedlots in southern Alberta. These were characterized by PFGE and antimicrobial susceptibility using a disk-diffusion assay. Select gene determinants were screened for using PCR. PFGE analysis revealed the isolates to be highly diverse. Ten percent of the isolates exhibited resistance. At present, the development and spread of antimicrobial resistance in M. haemolytica observed within the feedlots examined appears to be low.en_US
dc.identifier.urihttps://hdl.handle.net/10133/2517
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2009en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectBovine pneumonic pasteurellosisen_US
dc.subjectPathogenic bacteriaen_US
dc.subjectCattle -- Pathogens -- Researchen_US
dc.subjectCattle -- Diseases -- Researchen_US
dc.subjectFeedlotsen_US
dc.subjectDissertations, Academicen_US
dc.titleCharacterization of the genetic diversity and antimicrobial resistance in Mannheimia haemolytica from feedlot cattleen_US
dc.typeThesisen_US
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