Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron

dc.contributor.authorSmith, Marshall B.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2019-06-19T21:48:04Z
dc.date.available2019-06-19T21:48:04Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractGenetically engineering symbiotic bacteria remains an underexploited opportunity to improve host-health and create new classes of biological devices, such as diagnostics or intestinal delivery systems for therapeutics. Bacteroides thetaiotamicron (B. theta) is a Gram-negative intestinal anaerobe with potential for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory fidelity of transgene expression in B. theta, I have developed platform expression strains with engineered regulatory proteins under control of promoter elements that respond to dextran and arabinogalactan, two chemically distinct glycans. In addition to single glycan induction, I have also developed a novel “dual-glycan” expression system that requires the addition of both dextran and arabinogalactan for induction. Additionally my engineered strains are compatible with a series of chromosomal integration and episomal vectors that improve the throughput of gene cloning, integration, and expression. Together this expression system provides a new collection of glycan-responsive tools to improve transgene expression in B. theta and provides the proof-of-concept for engineering more complex dual-glycan expression systems.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5416
dc.language.isoen_USen_US
dc.proquest.subject0410en_US
dc.proquest.subject0369en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectBacteroidesen_US
dc.subjectBacterial genetic engineeringen_US
dc.subjectIntestines -- Microbiologyen_US
dc.subjectMicrobial biotechnologyen_US
dc.subjectArabinogalactanen_US
dc.subjectDextranen_US
dc.subjectBacteroidetesen_US
dc.subjectdistal gut microbiotaen_US
dc.subjectgenetically-engineered bacteriaen_US
dc.subjectprobiotic engineeringen_US
dc.subjectDissertations, Academicen_US
dc.titleEngineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicronen_US
dc.typeThesisen_US
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