Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron

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dc.contributor.authorSmith, Marshall B.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2019-06-19T21:48:04Z
dc.date.available2019-06-19T21:48:04Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractGenetically engineering symbiotic bacteria remains an underexploited opportunity to improve host-health and create new classes of biological devices, such as diagnostics or intestinal delivery systems for therapeutics. Bacteroides thetaiotamicron (B. theta) is a Gram-negative intestinal anaerobe with potential for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory fidelity of transgene expression in B. theta, I have developed platform expression strains with engineered regulatory proteins under control of promoter elements that respond to dextran and arabinogalactan, two chemically distinct glycans. In addition to single glycan induction, I have also developed a novel “dual-glycan” expression system that requires the addition of both dextran and arabinogalactan for induction. Additionally my engineered strains are compatible with a series of chromosomal integration and episomal vectors that improve the throughput of gene cloning, integration, and expression. Together this expression system provides a new collection of glycan-responsive tools to improve transgene expression in B. theta and provides the proof-of-concept for engineering more complex dual-glycan expression systems.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5416
dc.language.isoen_USen_US
dc.proquest.subject0410en_US
dc.proquest.subject0369en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectBacteroidesen_US
dc.subjectBacterial genetic engineeringen_US
dc.subjectIntestines -- Microbiologyen_US
dc.subjectMicrobial biotechnologyen_US
dc.subjectArabinogalactanen_US
dc.subjectDextranen_US
dc.subjectBacteroidetesen_US
dc.subjectdistal gut microbiotaen_US
dc.subjectgenetically-engineered bacteriaen_US
dc.subjectprobiotic engineeringen_US
dc.subjectDissertations, Academicen_US
dc.titleEngineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicronen_US
dc.typeThesisen_US
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