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dc.contributor.author Reti, Kristen L.
dc.contributor.author Thomas, Matthew C.
dc.contributor.author Yanke, L. Jay
dc.contributor.author Selinger, L. Brent
dc.contributor.author Inglis, G. Douglas
dc.date.accessioned 2019-06-12T20:52:36Z
dc.date.available 2019-06-12T20:52:36Z
dc.date.issued 2013
dc.identifier.citation Reti, K. L., Thomas, M. C., Yanke, L. J., Selinger, L. B., & Inglis, G. D. (2013). Effect of antimicrobial growth promoter administration on the intestinal microbiota of beef cattle. Gut Pathogens, 5(8). https://doi.org/10.1186/1757-4749-5-8 en_US
dc.identifier.uri https://hdl.handle.net/10133/5395
dc.description Sherpa Romeo green journal. Open access journal. Creative Commons Attribution 2.0 Generic License (CC BY 2.0) applies en_US
dc.description.abstract Background: Antimicrobial growth promoters (AGPs) are antimicrobial agents administered to livestock in feed for prolonged periods to enhance feed efficiency. Beef cattle are primarily finished in confined feeding operations in Canada and the USA, and the administration of AGPs such as chlortetracycline and sulfamethazine (Aureo S-700 G) is the standard. The impacts of AGPs on the intestinal microbiota of beef cattle are currently uncertain; it is documented that AGPs administered to beef cattle pass through the rumen and enter the intestine. To ascertain the impacts of Aureo S-700 G on the small and large intestinal microbiota of beef cattle (mucosa-associated and within digesta), terminal restriction fragment length polymorphism (T-RFLP) analysis and quantitative PCR (qPCR) for total bacteria were applied. Beef cattle were maintained in an experimental feedlot (five replicate pens per treatment), and AGP treatment cattle were administered Aureo S-700 G in feed, whereas control cattle were administered no antimicrobials. As the intestinal microbiota of beef cattle has not been extensively examined, clone library analysis was applied to ascertain the primary bacterial constituents of the intestinal microbiota. Results: Comparative T-RFLP and qPCR analysis (n = 122 samples) revealed that bacterial community fingerprints and bacterial load within digesta differed from those associated with mucosa. However, the administration of Aureo S-700 G did not affect bacterial community fingerprints or bacterial load within the small and large intestine relative to control cattle. Analysis of >1500 near full length 16S rDNA clones revealed considerably greater bacterial diversity in the large relative to the small intestine of beef cattle. Mucosa-associated bacterial communities in the jejunum were dominated by Proteobacteria, and differed conspicuously from those in the ileum and large intestine. Although the ileum contained bacterial clones that were common to the jejunum as well as the cecum, Firmicutes clones associated with mucosa dominated in the ileum, cecum, and descending colon. In the descending colon, clone library analysis did not reveal a difference in the richness or diversity of bacterial communities within digesta relative to those associated with mucosa. However, T-RFLP analysis indicated a significant difference in T-RF relative abundance (i.e. difference in relative taxon abundance) between mucosa-associated and digesta communities attributed in part to the differential abundance of Bacteriodes, Alistipes, Oscillibacter, and unclassified Clostridiales. Conclusions: These data demonstrate that there was no significant difference in the composition of the predominant intestinal bacteria constituents within animals administered Aureo S-700 G and those not administered AGPs after a 28 day withdrawal period. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.subject Antimicrobial growth promoters en_US
dc.subject AGP en_US
dc.subject Beef cattle en_US
dc.subject Intestine en_US
dc.subject Microbiota en_US
dc.subject Intestinal bacteria
dc.subject.lcsh Anti-infective agents
dc.title Effect of antimicrobial growth promoter administration on the intestinal microbiota of beef cattle en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution Agriculture and Agri-Food Canada Research Centre en_US
dc.publisher.institution University of Lethbridge en_US


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