dc.contributor.author |
Munns, Krysty D. |
|
dc.contributor.author |
Zaheer, Rahat |
|
dc.contributor.author |
Xu, Yong |
|
dc.contributor.author |
Stanford, Kim |
|
dc.contributor.author |
Laing, Chad R. |
|
dc.contributor.author |
Gannon, Victor P. J. |
|
dc.contributor.author |
Selinger, L. Brent |
|
dc.contributor.author |
McAllister, Tim A. |
|
dc.date.accessioned |
2017-01-16T22:03:07Z |
|
dc.date.available |
2017-01-16T22:03:07Z |
|
dc.date.issued |
2016 |
|
dc.identifier.citation |
Munns, K. D., Zaheer, R., Xu, Yohg, Stanford, K., Laing, C. R., Gannon, V. P. J., ... McAllister, T. A. (2016). Comparative genomic analysis of Escherichia coli 0157:H7 isolated from super-shedder and low-shedder cattle. PLoS ONE, 11(3), e0151673. doi:10.1371/journal.pone.0151673 |
en_US |
dc.identifier.uri |
https://hdl.handle.net/10133/4760 |
|
dc.description |
Sherpa Romeo green journal; open access |
en_US |
dc.description.abstract |
Cattle are the primary reservoir of the foodborne pathogen Escherichia coli O157:H7, with
the concentration and frequency of E. coli O157:H7 shedding varying substantially among
individual hosts. The term ‘‘super-shedder” has been applied to cattle that shed 104 cfu E.
coli O157:H7/g of feces. Super-shedders have been reported to be responsible for the
majority of E. coli O157:H7 shed into the environment. The objective of this study was to
determine if there are phenotypic and/or genotypic differences between E. coli O157:H7 isolates
obtained from super-shedder compared to low-shedder cattle. From a total of 784 isolates,
four were selected from low-shedder steers and six isolates from super-shedder
steers (4.01–8.45 log cfu/g feces) for whole genome sequencing. Isolates were phage and
clade typed, screened for substrate utilization, pH sensitivity, virulence gene profiles and
Stx bacteriophage insertion (SBI) sites. A range of 89–2473 total single nucleotide polymorphisms
(SNPs) were identified when sequenced strains were compared to E. coli O157:H7
strain Sakai. More non-synonymous SNP mutations were observed in low-shedder isolates.
Pan-genomic and SNPs comparisons did not identify genetic segregation between supershedder
or low-shedder isolates. All super-shedder isolates and 3 of 4 of low-shedder isolates
were typed as phage type 14a, SBI cluster 3 and SNP clade 2. Super-shedder isolates
displayed increased utilization of galactitol, thymidine and 3-O-β-D-galactopyranosyl-Darabinose
when compared to low-shedder isolates, but no differences in SNPs were
observed in genes encoding for proteins involved in the metabolism of these substrates.
While genetic traits specific to super-shedder isolates were not identified in this study, differences
in the level of gene expression or genes of unknown function may still contribute to some strains of E. coli 0157:H7 reaching high densities within bovine feces. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
Public Library of Science |
en_US |
dc.subject |
Escherichia coli 0157:H7 |
en_US |
dc.subject |
E. coli |
en_US |
dc.subject |
Super-shedder cattle |
en_US |
dc.subject |
Low-shedder cattle |
en_US |
dc.subject |
Cattle -- Feces |
en_US |
dc.title |
Comparative genomic analysis of Escherichia coli 0157:H7 isolated from super-shedder and low-shedder cattle |
en_US |
dc.type |
Article |
en_US |
dc.publisher.faculty |
Arts and Science |
en_US |
dc.publisher.department |
Department of Biological Sciences |
en_US |
dc.description.peer-review |
Yes |
en_US |
dc.publisher.institution |
Lethbridge Research Centre |
en_US |
dc.publisher.institution |
University of Lethbridge |
en_US |
dc.publisher.institution |
Agriculture and Forestry |
en_US |
dc.publisher.institution |
Public Health Agency of Canada |
en_US |