Faculty Research and Publications

Recent Submissions

  • Bressler, Kamiko R.; Ross, Joseph A.; Ilnytskyy, Slava; Vanden Dungen, Keiran; Taylor, Katrina; Patel, Kush; Zovoilis, Athanasios; Kovalchuk, Igor; Thakor, Nehal (SpringerArts and ScienceDepartment of Chemistry and BiochemistryDepartment of Biological SciencesDepartment of NeuroscienceUniversity of LethbridgeUniversity of Calgaryhttps://doi.org/10.1007/s12192-020-01174-1, 2020)
    During the integrated stress response (ISR), global translation initiation is attenuated; however, noncanonical mechanisms allow for the continued translation of specific transcripts. Eukaryotic initiation factor 5B (eIF5B) ...
  • Ross, Joseph A.; Ahn, Bo Young; King, Jennifer; Bressler, Kamiko R.; Senger, Donna L.; Thakor, Nehal (Canadian Science PublishingArts and ScienceDepartment of Chemistry and BiochemistryDepartment of NeuroscienceUniversity of LethbridgeUniversity of Calgaryhttps://doi.org/10.1139/bcb-2019-0329, 2019)
    Glioblastoma multiforme (GBM) is among the deadliest cancers, owing in part to complex inter- and intra-tumor heterogeneity and the presence of a population of stem-like cells called brain tumor stem cells (BTSCs/BTICs). ...
  • Balasingam, Nirujah; Brandon, Harland E.; Ross, Joseph A.; Wieden, Hans-Joachim; Thakor, Nehal (Canadian Science PublishingArts and ScienceAlberta RNA Research and Training (ARRTI)Department of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1139/bcb-2018-0353, 2020)
    P-loop NTPases comprise one of the major superfamilies of nucleotide binding proteins, which mediate a variety of cellular processes, such as mRNA translation, signal transduction, cell motility, and growth regulation. In ...
  • Nshogozabahizi, J. C.; Aubrey, K. L.; Ross, J. A.; Thakor, Nehal (WileyArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1111/jam.14270, 2019)
    Synthetic biology requires the design and implementation of novel enzymes, genetic circuits or even entire cells, which can be controlled by the user. RNA‐based regulatory elements have many important functional properties ...
  • Trofimenkoff, Elizabeth A. M.; Roussel, Marc R. (ElsevierArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1016/j.mbs.2020.108376, 2020)
    During the templated biopolymerization processes of transcription and translation,a macro molecular machine, either an RNA polymerase or a ribosome, binds to a specific site on the template. Due to the sizes of these ...
  • Fernandes, Dennis D.; Bamrah, Jasbir; Kailasam, Senthilkumar; Gomes, Gregory-Neal W.; Li, Yuchong; Wieden, Hans-Joachim; Gradinaru, Claudiu C. (Nature ResearchArts and ScienceDepartment of Chemistry and BiochemistryUniversity of TorontoUniversity of Lethbridgehttps://doi.org/10.1038/s41598-017-13427-8, 2017)
    In recent years, new labelling strategies have been developed that involve the genetic insertion of small amino-acid sequences for specific attachment of small organic fluorophores. Here, we focus on the tetracysteine FCM ...
  • Mercier, Evan; Girodat, Dylan; Wieden, Hans-Joachim (Nature ResearchArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1038/srep07677, 2015)
    The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding ...
  • De Laurentiis, Evelina I.; Wieden, Hans-Joachim (Nature ResearchArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://dx.doi.org/10.1038/srep08573, 2015)
    The bacterial translational GTPase EF4/LepA is structurally similar to the canonical elongation factor EF-G. While sharing core structural features with other translational GTPases, the function of EF4 remains unknown. ...
  • Roussel, Marc R. (SpringerArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1007/978-3-030-11554-8_14, 2019)
    In Escherichia coli, an enzyme called Hmp is a key contributor to the detoxification of nitric oxide (NO). In the absence of NO, the transcription of the hmp gene is repressed by an iron-sulfur protein called FNR. NO damages ...
  • De Laurentiis, Evelina I.; Mercier, Evan; Wieden, Hans-Joachim (American Society for Biochemistry and Molecular BiologyArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://dx.doi.org/10.1074/jbc.M116.740381, 2015)
    Little is known about the conservation of critical kinetic parameters and the mechanistic strategies of elongation factor (EF) Ts-catalyzed nucleotide exchange in EF-Tu in bacteria and particularly in clinically relevant ...
  • Tanzawa, Takehito; Kato, Koji; Girodat, Dylan; Ose, Toyoyuki; Kumakura, Yuki; Weiden, Hans-Joachim; Uchiumi, Toshio; Tanaka, Isao; Yao, Min (Oxford University PressArts and ScienceDepartment of Chemistry and BiochemistryHokkaido UniversityUniversity of LethbridgeNiigata Universityhttps://doi.org/10.1093/nar/gky115, 2018)
    Archaea and eukaryotes have ribosomal P stalks composed of anchor protein P0 and aP1 homodimers (archaea) or P1•P2 heterodimers (eukaryotes). These P stalks recruit translational GTPases to the GTPase-associated center in ...
  • Roussel, Marc R. (ElsevierArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1016/j.mbs.2019.108274, 2019)
    Volume 1, Issue 1 of Mathematical Biosciences was the venue for a now-classic paper on the application of singular perturbation theory in enzyme kinetics, “On the mathematical status of the pseudo-steady state hypothesis ...
  • El Bourakadi, Khadija; El Mehdi Mekhzoum, Mohamed; Boeré, René T.; El Kacem Qais, Abou; Bouhfid, Rachid (ElsevierArts and ScienceDepartment of Chemistry and BiochemistryInstitute of Nanomaterial and Nanotechnology (NANOTECH)Mohammed V-Rabat UniversityUniversity of Lethbridgehttps://doi.org/10.1016/j.molstruc.2019.127253, 2019)
    6-bis(2-(thiazol-4-yl)-benzimidazol-1-yl)hexane has been efficiently synthesized by direct alkylation of 2-(4-thiazolyl)benzimidazole with 1,6-dibromohexane via phase-transfer catalysis. The desired compound was obtained ...
  • Becke, A. D.; Roussel, Marc R. (American Physical SocietyArts and ScienceDepartment of Chemistry and BiochemistryQueen's UniversityUniversity of Lethbridgehttps://doi.org/10.1103/PhysRevA.39.3761, 1989)
    We present a new coordinate-space model of spherically averaged exchange-hole functions in inhomogeneous systems that depends on local values of the density and its gradient and Laplacian, and also the kinetic energy ...
  • Beekman, Richard A.; Roussel, Marc R.; Wilson, P. J. (American Physical SocietyArts and ScienceDepartment of Chemistry and BiochemistryDepartment of PhysicsUniversity of Lethbridgehttps://doi.org/10.1103/PhysRevA.59.503, 1999)
    We have computed the equilibrium conformations of clusters of up to 100 ions in a spherically symmetric harmonic trap by a simple optimization strategy called seeding in which ions are added or removed from ...
  • Roussel, Marc R.; Wang, Jichang (American Physical SocietyArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1103/PhysRevLett.87.188302, 2001)
    In this Letter, we report the observation of a transition from self-replicating behavior to stationary spatial structures induced by concentration-dependent diffusivities in the excitable Gray-Scott medium. Notably, the ...
  • Closson, Taunia L. L.; Roussel, Marc R. (American Physical SocietyArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridgehttps://doi.org/10.1103/PhysRevLett.85.3974, 2000)
    Many natural and technological systems have on/off switches. For instance, mitosis can be halted by biochemical switches which act through the phosphorylation state of a complex called mitosis promoting factor. If switching ...
  • Juranek, Stefan; Wieden, Hans-Joachim; Lipps, Hans J. (Oxford University PressArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Witten/HerdeckeUniversity of Lethbridgehttps://doi.org/10.1093/nar/gkg233, 2003)
    Dramatic DNA reorganization and elimination processes occur during macronuclear differentiation in ciliates. In this study we analyzed whether cytosine methylation of specific sequences plays a functional role during DNA ...
  • Gu, Shan-Qing; Peske, Frank; Wieden, Hans-Joachim; Rodnina, Marina V.; Wintermeyer, Wolfgang (Cold Springs Harbor Laboratory PressArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Witten/HerdeckeUniversity of Lethbridgehttps://dx.doi.org/10.1261/rna.2196403, 2003)
    The signal recognition particle (SRP) from Escherichia coli, composed of Ffh protein and 4.5S RNA, mediates membrane targeting of translating ribosomes displaying a signal or signal-anchor sequence. SRP binds at the peptide ...
  • Armstrong, A.; Chivers, T.; Boeré, René T. (American Chemical SocietyArts and ScienceDepartment of Chemistry and BiochemistryUniversity of CalgaryUniversity of Lethbridge, 2005)
    Stable and persistent phosphorus-containing radicals can be divided into five general categories: phosphinyl, diphosphanyl, 1,3-diphosphaallyl, lithiated tetrakisimidophosphates, and phosphaverdazyl systems. The syntheses, ...

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