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dc.contributor.supervisor Golsteyn, Roy
dc.contributor.author Lewis, Cody W.
dc.date.accessioned 2014-10-03T20:13:08Z
dc.date.available 2014-10-03T20:13:08Z
dc.date.issued 2014-10-03
dc.identifier.uri https://hdl.handle.net/10133/3505
dc.description.abstract This thesis examines the relationship between checkpoint adaptation and micronuclei in human cancer cells with damaged DNA. When cancer cells are exposed to genotoxic agents they can form micronuclei, although the precise process has yet to be described. I examined glioma cells (M059K) and found that 48% of the cells contain micronuclei after treatment with cisplatin. Treated cells also underwent checkpoint adaptation: they signalled damaged DNA, had active Chk1, arrested in G2-phase, and then entered mitosis. The relationship between checkpoint adaptation and micronuclei was confirmed by use of chemical inhibitors against Chk1 or Cdk1, which have key roles in checkpoint adaptation. When Chk1 was inhibited in treated cells, additional micronuclei formed, whereas if Cdk1 was inhibited, no additional micronuclei formed. I treated normal cells (WI-38) with cisplatin and found that they did not undergo checkpoint adaptation or form micronuclei. These data demonstrate that micronuclei are an outcome of checkpoint adaptation. en_US
dc.description.sponsorship Alberta Innovates Technology Futures, Alberta Scholarship Programs, University of Lethbridge School of Graduate Studies en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences.
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science)
dc.subject cancer cells
dc.subject checkpoint adaptation
dc.subject genotoxic agents
dc.subject glioma cells
dc.subject micronuclei
dc.subject normal cells
dc.title Testing the relationship between checkpoint adaptation and micronuclei in human fibroblastic glioma and normal lung cells en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquest.subject 0379 en_US
dc.proquest.subject 0992
dc.proquest.subject 0419
dc.proquestyes Yes en_US


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