The progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modifications and gene expression

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dc.contributor.authorBilichak, Andriy
dc.contributor.authorIlnytskyy, Yaroslav
dc.contributor.authorHollunder, Jens
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2016-10-27T02:43:48Z
dc.date.available2016-10-27T02:43:48Z
dc.date.issued2012
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractPlants are able to acclimate to new growth conditions on a relatively short time-scale. Recently, we showed that the progeny of plants exposed to various abiotic stresses exhibited changes in genome stability, methylation patterns and stress tolerance. Here, we performed a more detailed analysis of methylation patterns in the progeny of Arabidopsis thaliana (Arabidopsis) plants exposed to 25 and 75 mM sodium chloride. We found that the majority of gene promoters exhibiting changes in methylation were hypermethylated, and this group was overrepresented by regulators of the chromatin structure. The analysis of DNA methylation at gene bodies showed that hypermethylation in the progeny of stressed plants was primarily due to changes in the 59 and 39 ends as well as in exons rather than introns. All but one hypermethylated gene tested had lower gene expression. The analysis of histone modifications in the promoters and coding sequences showed that hypermethylation and lower gene expression correlated with the enrichment of H3K9me2 and depletion of H3K9ac histones. Thus, our work demonstrated a high degree of correlation between changes in DNA methylation, histone modifications and gene expression in the progeny of salt-stressed plants.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBilichak, A., Ilnystkyy, Y., Hollunder, J., & Kovalchuk, I. (2012). The progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modifications and gene expression. PLoS ONE, 7(1), e30515. doi:10.1371/journal.pone.0030515en_US
dc.identifier.urihttps://hdl.handle.net/10133/4629
dc.language.isoen_CAen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionGhent Universityen_US
dc.subjectDNA methylationen_US
dc.subjectHistone modificationsen_US
dc.subjectGene expressionen_US
dc.subjectArabidopsis thalianaen_US
dc.subjectStressed plantsen_US
dc.subjectStress toleranceen_US
dc.subjectProgenyen_US
dc.subjectSalt-stressed plantsen_US
dc.titleThe progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modifications and gene expressionen_US
dc.typeArticleen_US
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