Quantitative surveillance of shiga toxins 1 and 2, Escherichia coli O178 and O157 in feces of western-Canadian slaughter cattle enumerated by droplet digital PCR with a focus on seasonality and slaughterhouse location

Simple item page
dc.contributor.authorPaquette, Sarah-Jo
dc.contributor.authorStanford, Kim
dc.contributor.authorThomas, James E.
dc.contributor.authorReuter, Tim
dc.date.accessioned2018-11-28T23:44:42Z
dc.date.available2018-11-28T23:44:42Z
dc.date.issued2018
dc.descriptionOpen access; distributed under the terms of the Creative Commons Attribution Licenseen_US
dc.description.abstractOften Escherichia coli are harmless and/or beneficial bacteria inhabiting the gastrointestinal tract of livestock and humans. However, Shiga toxin-producing E. coli (STEC) have been linked to human disease. Cattle are the primary reservoir for STEC and STEC “super-shedders” are considered to be a major contributor in animal to animal transmission. Among STEC, O157:H7 is the most recognized serotype, but in recent years, non-O157 STEC have been increasingly linked to human disease. In Argentina and Germany, O178 is considered an emerging pathogen. Our objective was to compare populations of E. coli O178, O157, shiga toxin 1 and 2 in western Canadian cattle feces from a sampling pool of ~80,000 beef cattle collected at two slaughterhouses. Conventional PCR was utilized to screen 1,773 samples for presence/absence of E. coli O178. A subset of samples (n = 168) was enumerated using droplet digital PCR (ddPCR) and proportions of O178, O157 and shiga toxins 1 & 2 specific-fragments were calculated as a proportion of generic E. coli (GEC) specific-fragments. Distribution of stx1 and stx2 was determined by comparing stx1, stx2 and O157 enumerations. Conventional PCR detected the presence of O178 in 873 of 1,773 samples and ddPCR found the average proportion of O178, O157, stx1 and stx2 in the samples 2.8%, 0.6%, 1.4% and 0.5%, respectively. Quantification of stx1 and stx2 revealed more virulence genes than could be exclusively attributed to O157. Our results confirmed the presence of E. coli O178 in western Canadian cattle and ddPCR revealed O178 as a greater proportion of GEC than was O157. Our results suggests: I) O178 may be an emerging subgroup in Canada and II) monitoring virulence genes may be a more relevant target for food-safety STEC surveillance compared to current serogroup screening.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationPaquette, S., Stanford, K., Thomas, J., & Reuter, T. (2018). Quantitative surveillance of shiga toxins 1 and 2, Escherichia coli O178 and O157 in feces of western-Canadian slaughter cattle enumerated by droplet digital PCR with a focus on seasonality and slaughterhouse location. PLoS ONE, 13(4), e0195880. https: doi.org/10.1371/journal.pone.0195880en_US
dc.identifier.urihttps://hdl.handle.net/10133/5254
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionAlberta Agriculture and Forestryen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectE. coli O178en_US
dc.subjectE. coli O157en_US
dc.subjectShiga toxinen_US
dc.subjectCattle fecesen_US
dc.subjectSlaughter cattleen_US
dc.subjectSlaughterhouseen_US
dc.subjectPathogensen_US
dc.subjectDroplet digital PCRen_US
dc.subject.lcshEscherichia coli
dc.subject.lcshCattle--Canada, Western
dc.subject.lcshPathogenic microorganisms
dc.subject.lcshSlaughtering and slaughtering-houses--Canada, Western
dc.titleQuantitative surveillance of shiga toxins 1 and 2, Escherichia coli O178 and O157 in feces of western-Canadian slaughter cattle enumerated by droplet digital PCR with a focus on seasonality and slaughterhouse locationen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thomas quantitative surveillance of shiga.pdf
Size:
2.39 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thomas, James