Evolutionary divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia

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dc.contributor.authorHudson, Andrew J.
dc.contributor.authorMoore, Ashley N.
dc.contributor.authorElniski, David
dc.contributor.authorJoseph, Joella
dc.contributor.authorYee, Janet
dc.contributor.authorRussell, Anthony G.
dc.date.accessioned2017-07-12T20:06:05Z
dc.date.available2017-07-12T20:06:05Z
dc.date.issued2012
dc.descriptionSherpa Romeo green journalen_US
dc.description.abstractNon-coding RNAs (ncRNAs) have diverse essential biological functions in all organisms, and in eukaryotes, two such classes of ncRNAs are the small nucleolar (sno) and small nuclear (sn) RNAs. In this study, we have identified and characterized a collection of sno and snRNAs in Giardia lamblia, by exploiting our discovery of a conserved 12 nt RNA processing sequence motif found in the 30 end regions of a large number of G. lamblia ncRNA genes. RNA end mapping and other experiments indicate the motif serves to mediate ncRNA 30 end formation from mono- and di-cistronic RNA precursor transcripts. Remarkably, we find the motif is also utilized in the processing pathway of all four previously identified trans-spliced G. lamblia introns, revealing a common RNA processing pathway for ncRNAs and trans-spliced introns in this organism. Motif sequence conservation then allowed for the bioinformatic and experimental identification of additional G. lamblia ncRNAs, including new U1 and U6 spliceosomal snRNA candidates. The U6 snRNA candidate was then used as a tool to identity novel U2 and U4 snRNAs, based on predicted phylogenetically conserved snRNA–snRNA base-pairing interactions, from a set of previously identified G. lamblia ncRNAs without assigned function. The Giardia snRNAs retain the core features of spliceosomal snRNAs but are sufficiently evolutionarily divergent to explain the difficulties in their identification. Most intriguingly, all of these snRNAs show structural features diagnostic of U2-dependent/major and U12-dependent/minor spliceosomal snRNAs.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationHudson, A.J., Moore, A.N., Elniski, D., Joseph, J., Yee, J., & Russell, A.G. (2012). Evolutionary divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia. Nucleic Acids Research, 40(2), 10995-11008. doi:10.1093/nar/gks887en_US
dc.identifier.urihttps://hdl.handle.net/10133/4862
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionAlberta RNA Research and Training Instituteen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionTrent Universityen_US
dc.subjectNon-coding RNAsen_US
dc.subjectncRNAsen_US
dc.subjectsnRNAsen_US
dc.subjectSpliceosomalen_US
dc.subject.lcshGiardia lambia
dc.subject.lcshNon-coding RNA
dc.titleEvolutionary divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lambliaen_US
dc.typeArticleen_US
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