Reproducibility of fluorescent expression from engineering biological constructs in E. coli

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dc.contributor.authorBeal, Jacob
dc.contributor.authorHaddock-Angelli, Traci
dc.contributor.authorGershater, Markus
dc.contributor.authorDe Mora, Kim
dc.contributor.authorLizarazo, Meagan
dc.contributor.authorHollenhorst, Jim
dc.contributor.authorRettberg, Randy
dc.contributor.authoriGEM Interlab Study Contributors
dc.date.accessioned2019-06-26T18:48:17Z
dc.date.available2019-06-26T18:48:17Z
dc.date.issued2016
dc.descriptionSherpa Romeo green journal. Open access article. Creative Commons Attribution 4.0 International License (CC BY 4.0) appliesen_US
dc.description.abstractWe present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E.coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBeal, J., Haddock-Angelli, T., Gershater, M., de Mora, K., Lizarazo, M., Hollenhorst, J., ...iGEM Interlab Study Contributors. (2016). Reproducibility of fluorescent expression from engineered biological constructs in E.coli. PLoS ONE, 11(6), e0157255. doi:10.1371/journal.pone.0150182en_US
dc.identifier.urihttps://hdl.handle.net/10133/5436
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionRaytheon BBN Technologiesen_US
dc.publisher.institutioniGEM Foundationen_US
dc.publisher.institutionSynthaceen_US
dc.publisher.institutionAgilenten_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://dx.doi.org/10.1371/journal.pone.0150182
dc.titleReproducibility of fluorescent expression from engineering biological constructs in E. colien_US
dc.typeArticleen_US
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