Transgenerational changes in the genome stability and methylation in pathogen-infected plants (virus-induced plant genome instability)

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dc.contributor.authorBoyko, Alexander
dc.contributor.authorKathiria, Palak
dc.contributor.authorZemp, Franz J.
dc.contributor.authorYao, Youli
dc.contributor.authorPogribny, Igor
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2016-11-02T18:09:36Z
dc.date.available2016-11-02T18:09:36Z
dc.date.issued2007
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractPreviously, we reported the generation of a virusinduced systemic signal that increased the somatic and meiotic recombination rates in tobacco mosaic virus (TMV)-infected tobacco plants. Here, we analyzed the progeny of plants that received the signal and found that these plants also have a higher frequency of rearrangements in the loci carrying the homology to LRR region of the gene of resistance to TMV (N-gene). Analysis of the stability of repetitive elements from Nicotiana tabacum loci and 5.8S ribosomal RNA loci did not show any changes. Further analysis of the changes in the progeny of infected plants revealed that they had substantially hypermethylated genomes. At the same time, loci-specific methylation analysis showed: (1) profound hypomethylation in several LRR-containing loci; (2) substantial hypermethylation of actin loci and (3) no change in methylation in the loci of repetitive elements from N. tabacum or 5.8S ribosomal RNA. Global genome hypermethylation of the progeny is believed to be part of a general protection mechanism against stress, whereas locus-specific hypomethylation is associated with a higher frequency of rearrangements. Increased recombination events combined with the specific methylation pattern induced by pathogen attack could be a sign of an adaptive response by plantsen_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBoyko, A., Kathiria, P., Zemp, F. J., Yao, Y., Pogribny, I., & Kovalchuk, I. (2007). Transgenerational changes in the genome stability and methylation in pathogen-infected plants (virus-induced plant genome instability). Nucleic Acids Research, 35(5). doi:10.1093/nar/gkm029.en_US
dc.identifier.urihttps://hdl.handle.net/10133/4645
dc.language.isoen_CAen_US
dc.publisherOxford University Pressen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionNational Center for Toxicological Researchen_US
dc.subjectInfected plantsen_US
dc.subjectTransgenerational changesen_US
dc.subjectGenome stabilityen_US
dc.subjectMethylationen_US
dc.subjectHypermethylationen_US
dc.subjectProgenyen_US
dc.titleTransgenerational changes in the genome stability and methylation in pathogen-infected plants (virus-induced plant genome instability)en_US
dc.typeArticleen_US
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