Methods for the design and analysis of analytical ultracentrifugation experiments

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dc.contributor.authorDemeler, Borries
dc.date.accessioned2024-08-15T22:11:01Z
dc.date.available2024-08-15T22:11:01Z
dc.date.issued2024
dc.descriptionOpen access article. Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license (CC BY-NC-ND 4.0) applies
dc.description.abstractAnalytical ultracentrifugation experiments play an integral role in the solution-phase characterization of biological macromolecules and their interactions. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Proteomelab XL-A or XL-I analytical ultracentrifuge and with a Beckman Optima AUC. Instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.
dc.description.peer-reviewYes
dc.identifier.citationDemeler, B. (2024). Methods for the design and analysis of analytical ultracentrifugation Experiments. Current Protocols, 4, Article e975. https://doi.org/10.1002/0471140864.ps0713s60
dc.identifier.urihttps://hdl.handle.net/10133/6858
dc.language.isoen
dc.publisherWiley
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Montana
dc.publisher.urlhttps://doi.org/10.1002/cpz1.974
dc.subject2-dimensional spectrum analysis
dc.subjectAAV capsid quantification
dc.subjectAbsorbance optics
dc.subjectAnalytical buoyant-density equilibrium
dc.subjectAnalytical ultracentrifugation
dc.subjectComposition analysis
dc.subjectCustom grid method
dc.subjectExperimental design
dc.subjectIntensity measurements
dc.subjectInteractions studies
dc.subjectMonte Carlo method
dc.subjectMulti-wavelength analytical ultracentrifugation
dc.subjectParametrically constrained spectrum analysis
dc.subjectSedimentation velocity
dc.subjectSolution studies
dc.subjectStoichiometry determination
dc.subjectUltraScan
dc.subjectvan Holde-Weischet analysis
dc.titleMethods for the design and analysis of analytical ultracentrifugation experiments
dc.typeArticle
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