A genome-wide association study to identify diagnostic markers for human pathogenic Campylobacter jejuni strains

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dc.contributor.authorBuchanan, Cody J.
dc.contributor.authorWebb, Andrew L.
dc.contributor.authorMutschall, Steven K.
dc.contributor.authorKruczkiewicz, Peter
dc.contributor.authorBarker, Dillon Oliver Reese
dc.contributor.authorHetman, Benjamin M.
dc.contributor.authorGannon, Victor P. J.
dc.contributor.authorAbbott, D. Wade
dc.contributor.authorThomas, James E.
dc.contributor.authorInglis, G. Douglas
dc.contributor.authorTaboada, Eduardo N.
dc.date.accessioned2018-11-02T17:51:48Z
dc.date.available2018-11-02T17:51:48Z
dc.date.issued2017
dc.descriptionSherpa Romeo green journal; open accessen_US
dc.description.abstractCampylobacter jejuni is a leading human enteric pathogen worldwide and despite an improved understanding of its biology, ecology, and epidemiology, limited tools exist for identifying strains that are likely to cause disease. In the current study, we used subtyping data in a database representing over 24,000 isolates collected through various surveillance projects in Canada to identify 166 representative genomes from prevalent C. jejuni subtypes for whole genome sequencing. The sequence data was used in a genome-wide association study (GWAS) aimed at identifying accessory gene markers associated with clinically related C. jejuni subtypes. Prospective markers (n=28) were then validated against a large number (n=3,902) of clinically associated and non-clinically associated genomes from a variety of sources. A total of 25 genes, including six sets of genetically linked genes, were identified as robust putative diagnostic markers for clinically related C. jejuni subtypes. Although some of the genes identified in this study have been previously shown to play a role in important processes such as iron acquisition and vitamin B5 biosynthesis, others have unknown function or are unique to the current study and warrant further investigation. As few as four of these markers could be used in combination to detect up to 90% of clinically associated isolates in the validation dataset, and such markers could form the basis for a screening assay to rapidly identify strains that pose an increased risk to public health. The results of the current study are consistent with the notion that specific groups of C. jejuni strains of interest are defined by the presence of specific accessory genes.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBuchanan, C. J., Webb, A. L., Mutschall, S. K., Kruczkiewicz, P., Barker, D. O. R., Hetman, B. M., ... Taboada, E. N. (2017). A genome-wide association study to identify diagnostic markers for human pathogenic Campylobacter jejuni strains. Frontiers in Microbiology, 8(1224). doi: 10.3389/fmicb.2017.01224en_US
dc.identifier.urihttps://hdl.handle.net/10133/5233
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionNational Microbiology Laboratory at Lethbridgeen_US
dc.publisher.institutionLethbridge Research and Development Centreen_US
dc.subjectCampylobacter jejunien_US
dc.subjectGenome sequenceen_US
dc.subjectGenome-wide association studyen_US
dc.subjectClinical associationen_US
dc.subjectMolecular marker discoveryen_US
dc.subjectLinkage analysisen_US
dc.subjectMolecular risk assessmenten_US
dc.subject.lcshGenomics
dc.subject.lcshLinkage (Genetics)
dc.subject.lcshCampylobacter infections
dc.titleA genome-wide association study to identify diagnostic markers for human pathogenic Campylobacter jejuni strainsen_US
dc.typeArticleen_US
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