Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer

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dc.contributor.authorKrishnan, Preethi
dc.contributor.authorGhosh, Sunita
dc.contributor.authorGraham, Kathryn
dc.contributor.authorMackey, John R.
dc.contributor.authorKovalchuk, Olga
dc.contributor.authorDamaraju, Sambasivarao
dc.date.accessioned2019-01-23T20:41:31Z
dc.date.available2019-01-23T20:41:31Z
dc.date.issued2016
dc.descriptionSherpa Romeo blue journal. Open access article. Creative Commons Attribution 3.0 License (CC BY 3.0) appliesen_US
dc.description.abstractPiwi-interacting RNAs (piRNAs), whose role in germline maintenance has been established, are now also being classified as post-transcriptional regulators of gene expression in somatic cells. PIWI proteins, central to piRNA biogenesis, have been identified as genetic and epigenetic regulators of gene expression. piRNAs/PIWIs have emerged as potential biomarkers for cancer but their relevance to breast cancer has not been comprehensively studied. piRNAs and mRNAs were profiled from normal and breast tumor tissues using next generation sequencing and Agilent platforms, respectively. Gene targets for differentially expressed piRNAs were identified from mRNA expression dataset. piRNAs and PIWI genes were independently assessed for their prognostic significance (outcomes: Overall Survival, OS and Recurrence Free Survival, RFS). We discovered eight piRNAs as novel independent prognostic markers and their association with OS was confirmed in an external dataset (The Cancer Genome Atlas). Further, PIWIL3 and PIWIL4 genes showed prognostic relevance. 306 gene targets exhibited reciprocal relationship with piRNA expression. Cancer cell pathways such as apoptosis and cell signaling were the key Gene Ontology terms associated with the regulated gene targets. Overall, we have captured the entire cascade of events in a dysregulated piRNA pathway and have identified novel markers for breast cancer prognostication.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationKrishnan, P., Ghosh, S., Graham, K., Mackey, J R., Kovalchuk, O., & Damaraju, S. (2016). Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer. Oncotarget, 7(25), 37944-37956. https://doi.org/10.18632/oncotarget.9272en_US
dc.identifier.urihttps://hdl.handle.net/10133/5272
dc.language.isoen_USen_US
dc.publisherImpact Journalsen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.publisher.institutionAlberta Health Servicesen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.18632/oncotarget.9272
dc.subjectpiRNAen_US
dc.subjectPIWIen_US
dc.subjectBreast canceren_US
dc.subjectPrognostic markeren_US
dc.subjectTCGAen_US
dc.subjectPiwi-interactingen_US
dc.subject.lcshGene expression
dc.subject.lcshPiwi genes
dc.subject.lcshBreast--Cancer
dc.subject.lcshBreast--Cancer--Research
dc.titlePiwi-interacting RNAs and PIWI genes as novel prognostic markers for breast canceren_US
dc.typeArticleen_US
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