Measuring compressibility in the optima AUC™ analytical ultracentrifuge

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dc.contributor.authorStoutjesdyk, Marielle
dc.contributor.authorBrookes, Emre
dc.contributor.authorHenrickson, Amy
dc.contributor.authorDemeler, Borries
dc.date.accessioned2021-07-06T19:03:21Z
dc.date.available2021-07-06T19:03:21Z
dc.date.issued2020
dc.descriptionAccepted author manuscript.en_US
dc.description.abstractA method is described to accurately measure the compressibility of liquids using an analytical ultracentrifuge. The method makes use of very large pressure gradients, which can be generated in the analytical ultracentrifuge at high speeds to induce a maximum compression signal. Taking advantage of the new Optima AUC, which offers 10 micron radial resolution, a novel calibration centerpiece for measuring rotor stretch, and a speed-ramping procedure, even the weak compressibility of liquids like water, typically considered to be incompressible, could be detected. A model using the standard expression for the secant-average bulk modulus describing the relative compression of a liquid in the analytical ultracentrifuge is derived. The model is a function of the loading volume and the hydrostatic pressure generated in the analytical ultracentrifuge, as well as the secant-average bulk modulus. The compressibility of water and toluene were measured and the linear secant-average bulk modulus and meniscus positions were fitted. In addition to the measurement of the compressibility of liquids, applications for this method include an improved prediction of boundary conditions for multi-speed analytical ultracentrifugation experiments to better describe highly heterogeneous systems with analytical speed-ramping procedures, and the prediction of radius-dependent density variations.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationStoutjesdyk, M., Brookes, E., Henrickson, A., & Demeler, B. (2020). Measuring compressibility in the optima AUC™ analytical ultracentrifuge. European Biophysics Journal, 49, 711-718. https://doi.org/10.1007/s00249-020-01482-5en_US
dc.identifier.urihttps://hdl.handle.net/10133/5945
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1007/s00249-020-01482-5en_US
dc.subjectAnalytical ultracentrifugeen_US
dc.subjectBulk modulusen_US
dc.subjectNumerical modelingen_US
dc.subjectOptima AUC
dc.subject.lcshCentrifuges
dc.subject.lcshCompressibility
dc.titleMeasuring compressibility in the optima AUC™ analytical ultracentrifugeen_US
dc.typeArticleen_US
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