Changes in bacterial community composition of Escherichia coli O157:H7 super-shedder cattle occur in the lower intestine

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dc.contributor.authorZaheer, Rahat
dc.contributor.authorBony-Dugat, Eric
dc.contributor.authorHolman, Devon
dc.contributor.authorCousteix, Elodie
dc.contributor.authorXu, Yong
dc.contributor.authorMunns, Krysty D.
dc.contributor.authorSelinger, Lorna J.
dc.contributor.authorBarbieri, Rutn
dc.contributor.authorAlexander, Trevor W.
dc.contributor.authorMcAllister, Tim A.
dc.contributor.authorSelinger, L. Brent
dc.date.accessioned2019-08-23T22:55:41Z
dc.date.available2019-08-23T22:55:41Z
dc.date.issued2017
dc.descriptionSherpa Romeo green journal. Open access article. Creative Commons Attribution 4.0 International License (CC BY 4.0) applies.en_US
dc.description.abstractEscherichia coli O157:H7 is a foodborne pathogen that colonizes ruminants. Cattle are considered the primary reservoir of E. coli O157:H7 with super-shedders, defined as individuals excreting>104 E. coli O157:H7 CFU g-1 feces. The mechanisms leading to the super-shedding condition are largely unknown. Here, we used 16S rRNA gene pyrosequencing to examine the composition of the fecal bacterial community in order to investigate changes in the bacterial microbiota at several locations along the digestive tract (from the duodenum to the rectal-anal junction) in 5 steers previously identified as super-shedders and 5 non-shedders. The overall bacterial community structure did not differ by E. coli O157:H7 shedding status; but several differences in the relative abundance of taxa and OTUs were noted between the two groups. The genus Prevotella was most enriched in the non-shedders while the genus Ruminococcus and the Bacteroidetes phylum were notably enriched in the super-shedders. There was greater bacterial diversity and richness in samples collected from the lower- as compared to the upper gastrointestinal tract (GI). The spiral colon was the only GI location that differed in terms of bacterial diversity between super-shedders and non-shedders. These findings reinforced linkages between E. coli O157:H7 colonization in cattle and the nature of the microbial community inhabiting the digestive tract of supershedders.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationZaheer, R., Dugat-Bony, E., Holman, D., Cousteix, E., Xu, Y., Munns, K.,...Selinger, L. B. (2017). Changes in bacterial community composition of Escherichia coli O157:H7 super-shedder cattle occur in the lower intestine. PLoS ONE, 12(1), e0170050. doi:10.1371/journal.pone.0170050en_US
dc.identifier.urihttps://hdl.handle.net/10133/5513
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionLethbridge Research and Development Centreen_US
dc.publisher.institutionUniversité Paris-Saclayen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://dx.doi.org/10.1371/journal.pone.0170050
dc.subjectE. colien_US
dc.subjectFecal bacterial communityen_US
dc.subjectSuper-sheddersen_US
dc.subjectNon-sheddersen_US
dc.subjectSteersen_US
dc.subject.lcshEscherichia coli O157:H7
dc.subject.lcshBacterial diversity
dc.subject.lcshBeef cattle
dc.titleChanges in bacterial community composition of Escherichia coli O157:H7 super-shedder cattle occur in the lower intestineen_US
dc.typeArticleen_US
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