Archael proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA

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dc.contributor.authorKamalampeta, Rajashekhar
dc.contributor.authorWieden-Kothe, Ute
dc.date.accessioned2017-07-13T22:48:00Z
dc.date.available2017-07-13T22:48:00Z
dc.date.issued2012
dc.descriptionSherpa Romeo green journal. Open access article. Creative Commons Attribution 4.0 International License (CC BY 4.0) appliesen_US
dc.description.abstractCbf5 is a pseudouridine synthase that usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins; however archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA. This guide-independent activity of Cbf5 is enhanced by proteins Nop10 and Gar1 which are also found in H/ACA small ribonucleoproteins. Here, we analyzed the specific contribution of Nop10 and Gar1 for Cbf5-catalyzed pseudouridylation of tRNA. Interestingly, both Nop10 and Gar1 not only increase Cbf5’s affinity for tRNA, but they also directly enhance Cbf5’s catalytic activity by increasing the kcat of the reaction. In contrast to the guide RNA-dependent reaction, Gar1 is not involved in product release after tRNA modification. These results in conjunction with structural information suggest that Nop10 and Gar1 stabilize Cbf5 in its active conformation; we hypothesize that this might also be true for guide-RNA dependent pseudouridine formation by Cbf5.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationKamalampeta, R., & Kothe, U. (2012). Archael proteins Nop 10 and Gar 1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA. Scientific Reports, 2, 663. doi:10.1038/srep00663en_US
dc.identifier.urihttps://hdl.handle.net/10133/4868
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectCbf5en_US
dc.subjectNop10en_US
dc.subjectGar1en_US
dc.subjectPseudouridylationen_US
dc.subjectCatalytic activityen_US
dc.subjectArchaealen_US
dc.subjectPseudouridine formationen_US
dc.subjecttRNA modificationen_US
dc.subject.lcshTransfer RNA
dc.subject.lcshPseudouridine
dc.subject.lcshCatalysis
dc.titleArchael proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNAen_US
dc.typeArticleen_US
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