The stimulatory effect of CaCl2, NaCl and NH4NO3 salts on the ssDNA-binding activity of RecA depends on nucleotide cofactor and buffer pH

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dc.contributor.authorZiemienowicz, Alicja
dc.contributor.authorSeyed Mohammad, Reza Rahavi
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2017-04-28T20:36:55Z
dc.date.available2017-04-28T20:36:55Z
dc.date.issued2011
dc.descriptionOpen access journalen_US
dc.description.abstractThe single-stranded DNA binding activity of the Escherichia coli RecA protein is crucial for homologous recombination to occur. This and other biochemical activities of ssDNA binding proteins may be affected by various factors. In this study, we analyzed the effect of CaCl2, NaCl and NH4NO3 salts in combination with the pH and nucleotide cofactor effect on the ssDNA-binding activity of RecA. The studies revealed that, in addition to the inhibitory effect, these salts exert also a stimulatory effect on RecA. These effects occur only under very strict conditions, and the presence or absence and the type of nucleotide cofactor play here a major role. It was observed that in contrast to ATP, ATPγS prevented the inhibitory effect of NaCl and NH4NO3, even at very high salt concentration. These results indicate that ATPγS most likely stabilizes the structure of RecA required for DNA binding, making it resistant to high salt concentrations.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationZiemienowicz, A., Seyed Mohammad, R. R., & Kovalchuk, I. (2011). The stimulatory effect of CaCl2, NaCl and NH4NO3 salts on the ssDNA-binding activity of RecA depends on nucleotide cofactor and buffer pH. BMB Reports, 44(5), 341-346. doi:10.5483/BMBRep.2011.44.5.341en_US
dc.identifier.urihttps://hdl.handle.net/10133/4837
dc.language.isoen_USen_US
dc.publisherKorean Society for Biochemistry and Molecular Biologyen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectDNA-protein interactionen_US
dc.subjectHomologous recombinationen_US
dc.subjectMono-/divalent ion saltsen_US
dc.subjectRecAen_US
dc.subjectSingle-stranded DNAen_US
dc.subject.lcshDNA-protein interactions
dc.subject.lcshProtein binding
dc.subject.lcshDNA
dc.subject.lcshSalt
dc.subject.lcshSalts
dc.subject.lcshAmmonium nitrate
dc.subject.lcshCalcium chloride
dc.titleThe stimulatory effect of CaCl2, NaCl and NH4NO3 salts on the ssDNA-binding activity of RecA depends on nucleotide cofactor and buffer pHen_US
dc.typeArticleen_US
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