2026-09-19T13:37:17.571782100Zhttps://opus.uleth.ca/server/oai/request
oai:opus.uleth.ca:10133/60662021-10-21T06:05:38Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository supervisor Kovalchuk, Igor supervisor Laurie, John author Jensen, Theia May author University of Lethbridge. Faculty of Arts and Science 2021-10-20T17:51:41Z 2021-10-20T17:51:41Z 2021 https://hdl.handle.net/10133/6066 Obtaining doubled haploids plants from isolated microspores is valuable for plant breeders. Microspores generally require stress treatment to enter androgenesis and produce doubled haploids. RNA-seq analysis was performed on cultured microspores that have been subjected to a three-week cold treatment and exposure to the histone deacetylase inhibitor Trichostatin A. Results point to dramatic transcriptional reprogramming after cold pre-treatment; upregulation in transcription, cytoplasmic turn over, signal transduction through kinase cascades, and chromatin remodelling supporting a change in cell fate. Cold-stressed microspores are then able to respond to TSA treatment with transient genome-wide upregulation of several thousand genes, not seen in unstressed microspores. Pathways upregulated include mitotic cell cycle phase transition genes, transcription, and DNA recombination and repair. These pathways may explain the increase in androgenesis seen after application of TSA. Results shed light on the behavior of wheat microspores in culture and pave the way for improved androgenesis and DH production. en_US doubled haploids Trichostatin A cold-stressed microspores androgenesis wheat microspores wheat transcriptome Plant genetics -- Research Haploidy -- Research Androgenesis in plants Plants -- Effect of cold on Histone deacetylase -- Inhibitors -- Research Wheat -- Genetics -- Research Plant spores -- Effect of cold on -- Research Dissertations, Academic The impact of cold and Trichostatin A treatment on the transcriptome of wheat microspores Thesis 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