2026-09-20T08:56:47.677925Zhttps://opus.uleth.ca/server/oai/requestoai:opus.uleth.ca:10133/56702020-01-23T07:05:52Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository
supervisor
Wieden, Hans-Joachim
author
Vigar, Justin R. J.
author
University of Lethbridge. Faculty of Arts and Science
2020-01-22T20:51:05Z
2020-01-22T20:51:05Z
2019
https://hdl.handle.net/10133/5670
Translation initiation rates are fine-tuned by altering interactions between the ribosome and the translation initiation region of an mRNA. In bacteria varying levels of RNA structure attenuate these interactions by masking the ribosome-binding site from the small ribosomal subunit. Recent studies have described diverse strategies for recruiting mRNA to the ribosome, and highlighted the contributions of ribosomal protein S1. Here, I provide evidence that the non-canonical initiation mechanism that governs translation of the rpsA mRNA, encoding ribosomal protein S1, contains a three-dimensional architecture that is required for efficient translation. Furthermore, S1 plays an essential role during the initiation phase of translation by recruiting mRNA to the ribosome—unfolding structured mRNAs, and allowing for correct start codon positioning on the ribosome. Combining crosslinking immunoprecipitation and high-throughput sequencing approaches revealed the extent of S1’s involvement in mRNA recruitment, while also highlighting a broader role as a regulator of many RNA classes.
en_US
RNA
Biophysics
Synthetic Biology
Ribosome
Translation Initiation
Engineering
RNA-seq
Dissertations, Academic
rpsA and ribosomal protein S1: investigating a non-canonical translation initiation element
Thesis
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URL
http://localhost:4000/bitstreams/80aa5c22-9265-4bc4-b828-70d3f2b107e2/download
File
MD5
c66f071b1891fd77f85a4e051ff2b875
6173881
application/pdf
VIGAR_JUSTIN_MSC_2019.pdf
URL
http://localhost:4000/bitstreams/5b48e0ac-d971-4ca1-ae70-26e8f1b7d38e/download
File
MD5
52d8a3d72ca5c29deb9fc549c7f0f13b
328021
text/plain
VIGAR_JUSTIN_MSC_2019.pdf.txt