2026-09-21T23:42:41.669773Zhttps://opus.uleth.ca/server/oai/request
oai:opus.uleth.ca:10133/51732018-07-18T22:07:52Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository supervisor Thakor, Nehal supervisor Wieden, Hans-Joachim author Balasingam, Nirujah author University of Lethbridge. Faculty of Arts and Science 2018-07-18T22:06:42Z 2018-07-18T22:06:42Z 2017 https://hdl.handle.net/10133/5173 hOLA1 is a purine nucleotide binding protein that belongs to the P-loop GTPase family. hOLA1 suppresses protein synthesis by limiting ternary complex formation thus promoting the integrated stress response (ISR). ISR determines cell fate by activating or inhibiting pro- and anti-apoptotic proteins depending on the type of the stress condition. ISR facilitates cell survival in response to mild stress and promotes apoptosis in response to chronic stress. Depletion of hOLA1 leads to increased cell survival and diminished ISR. This study investigates the role of hOLA1 in apoptosis. My hypothesis was that diminished ISR in hOLA1-depleted cells leads to upregulation of anti-apoptotic proteins thus inhibiting apoptosis. Indeed, this study shows that hOLA1 depletion upregulates anti-apoptotic proteins such as cIAP1, cIAP2, and Bcl-xL, thus inhibiting apoptosis. The inhibition of apoptosis was studied by assessing caspase activation, cleaved poly (ADP-ribose) polymerase (PARP) level, and measuring apoptosis by propidium iodide (PI) staining and flow cytometry. en_US Adenosine triphosphatase Apoptosis Purine nucleotides Protein binding Breast -- Cancer Genetic translation breast cancer cancer hOLA1 translation Elucidating the role of human obg like ATPase1 (hOLA1) in apoptosis Thesis 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URL http://localhost:4000/bitstreams/a6634b72-b1c3-4aaf-bac5-1e215dc03d81/download File MD5 406170a4ddcc591b25ef203e94bb4cef 2042017 application/pdf BALASINGAM_NIRUJAH_MSC_2017.pdf URL http://localhost:4000/bitstreams/142be285-24c9-4850-9641-dfd6328ceee1/download File MD5 cb6c1c06cbc97e25eac7596b411d8d4a 141344 text/plain BALASINGAM_NIRUJAH_MSC_2017.pdf.txt