2026-09-20T05:26:00.735815400Zhttps://opus.uleth.ca/server/oai/request
oai:opus.uleth.ca:10133/45322018-08-07T21:09:16Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository supervisor Eudes, François supervisor Kovalchuk, Igor author Pepper, Jordan Tan author University of Lethbridge. Faculty of Arts and Science 2016-07-04T17:00:44Z 2016-07-04T17:00:44Z 2015 https://hdl.handle.net/10133/4532 Cell penetrating peptides (CPPs) are short 8-30 amino acid polypeptides that are able to transit across the semi-permeable plasma membrane of living cells, and deliver bound cargoes to the cytoplasm and other subcellular locations (e.g. nucleus, plastid organelles etc). The use of CPPs in plant cells and tissues remains a small and little researched field compared with the greater body of animal based research imperatives. The present research describes firstly, the measured interaction of the chosen model CPP Tat¬2 with the cell wall of microspores (i.e. microspore exine), secondly the reduction of the size and increase in regularity (measured as polydispersity) of Tat2 nucleic acid polyplexes and the unique empirical derivation of pseudo-kinetic parameters of nucleic acid CPP aggregation. Additionally several attempts to apply the findings from this research in plant cells and tissues are presented and discussed throughout. en_CA Cell-penetrating peptides Zeta-potential Dynamic light scattering Triticale siRNA dsRNA Microspore Characterization of cell-penetrating peptide complexation and interaction with plant cells Thesis 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 URL http://localhost:4000/bitstreams/2e2ebcf8-e7a9-4f37-8ba5-661ae803a58d/download File MD5 f650f80ae393bd0361071a27ee42eecc 1515039 application/pdf PEPPER_JORDAN_MSC_2015.pdf URL http://localhost:4000/bitstreams/46a08744-b719-4554-a6d6-f55352672aaa/download File MD5 628309b81f0b08075bd3e925882b4a62 195472 text/plain PEPPER_JORDAN_MSC_2015.pdf.txt