2026-09-18T19:59:56.554659100Zhttps://opus.uleth.ca/server/oai/requestoai:opus.uleth.ca:10133/36512018-08-01T21:03:55Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository
supervisor
Thomas, James E.
supervisor
Reuter, Tim
author
Conrad, Cheyenne
2015-03-31T20:32:37Z
2015-03-31T20:32:37Z
2014
https://hdl.handle.net/10133/3651
Shiga toxin-producing Escherichia coli (STEC) are human pathogens responsible for deadly outbreaks throughout the world. Cattle are the primary reservoir for these pathogens, secreting STEC in the feces. Bovine feces is the principal source of contamination of food products, however detection of STEC in feces has several inherent limitations. The objectives of this study were to develop and refine detection methods for STEC in cattle feces. Multiplex PCRs for detection of STEC serogroups and virulence genes were designed. Serogroup detection in feces significantly improved with enrichment (10 CFU/g feces). However, isolation of target serogroups from feces remains challenging, and additional refinements to immunomagnetic separation (IMS) techniques would be an asset. Novel techniques for detection and isolation of STEC across the farm-to-fork spectrum will continue to improve food safety monitoring and reduce risks to human health by decreasing circulation of contaminated products, aiding in diagnostics, and supporting outbreak management.
en_CA
Shiga toxin-producing Escherichia coli (STEC)
foodborne pathogen
food safety
Monitoring pathogenic Escherichia coli in Alberta beef cattle
Thesis
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URL
http://localhost:4000/bitstreams/a67761c3-d40b-457b-9688-11e76b6e2ca5/download
File
MD5
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application/pdf
CONRAD_CHEYENNE_MSC_2015.pdf
URL
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CONRAD_CHEYENNE_MSC_2015.pdf.txt