2026-09-20T04:37:02.201863700Zhttps://opus.uleth.ca/server/oai/requestoai:opus.uleth.ca:10133/26152019-02-07T06:06:54Zcom_10133_3346com_10133_1com_10133_296col_10133_3347col_10133_298
ULethbridge OPUS Repository
supervisor
Kovalchuk, Olga
author
McRae, Dorothy A.
author
University of Lethbridge. Faculty of Arts and Science
2012-02-24T19:11:14Z
2012-02-24T19:11:14Z
2010
https://hdl.handle.net/10133/2615
Most breast cancer patients undergo radiation diagnostics and are also treated with radiotherapy. In addition to being an important treatment modality, ionizing radiation (IR) is a potent tumour-causing agent that has been linked to breast cancer development. However, the exact molecular etiology of IR-induced mammary gland carcinogenesis remains unknown.
We set out to analyze the role of DNA methylation in mammary gland responses to low dose IR using a well-established rat model. We also studied low dose IR effects on global gene expression and microRNAome. We found that exposure to low, mammography-like dose of IR led to a significant loss of global DNA methylation in rat mammary gland tissue. Furthermore, low dose IR significantly affected rat mammary gland transcriptome and microRNAome.
The datasets generated within the scope of this thesis may be used to identify novel predictive biomarkers for assessment of the magnitude of IR effects on mammary gland tissue.
en_US
Ionizing radiation -- Health aspects
Ionizing radiation -- Physiological effect
Ionizing radiation -- Dose-response relationship
DNA -- Methylation
Breast -- Cancer -- Radiotherapy
Dissertations, Academic
Radiation induced epigenetic dysregulation in rat mammary gland tissue / Dorothy A. McRae
Thesis
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MCRAE_DOROTHY_MSC_2010.pdf.txt