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dc.contributor.supervisor Kovalchuk, Igor
dc.contributor.supervisor Laurie, John
dc.contributor.author Jensen, Theia May
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2021-10-20T17:51:41Z
dc.date.available 2021-10-20T17:51:41Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/10133/6066
dc.description.abstract Obtaining doubled haploids plants from isolated microspores is valuable for plant breeders. Microspores generally require stress treatment to enter androgenesis and produce doubled haploids. RNA-seq analysis was performed on cultured microspores that have been subjected to a three-week cold treatment and exposure to the histone deacetylase inhibitor Trichostatin A. Results point to dramatic transcriptional reprogramming after cold pre-treatment; upregulation in transcription, cytoplasmic turn over, signal transduction through kinase cascades, and chromatin remodelling supporting a change in cell fate. Cold-stressed microspores are then able to respond to TSA treatment with transient genome-wide upregulation of several thousand genes, not seen in unstressed microspores. Pathways upregulated include mitotic cell cycle phase transition genes, transcription, and DNA recombination and repair. These pathways may explain the increase in androgenesis seen after application of TSA. Results shed light on the behavior of wheat microspores in culture and pave the way for improved androgenesis and DH production. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject doubled haploids en_US
dc.subject Trichostatin A en_US
dc.subject cold-stressed microspores en_US
dc.subject androgenesis en_US
dc.subject wheat microspores en_US
dc.subject wheat transcriptome en_US
dc.subject Plant genetics -- Research en_US
dc.subject Haploidy -- Research en_US
dc.subject Androgenesis in plants en_US
dc.subject Plants -- Effect of cold on en_US
dc.subject Histone deacetylase -- Inhibitors -- Research en_US
dc.subject Wheat -- Genetics -- Research en_US
dc.subject Plant spores -- Effect of cold on -- Research en_US
dc.subject Dissertations, Academic en_US
dc.title The impact of cold and Trichostatin A treatment on the transcriptome of wheat microspores en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquest.subject 0369 en_US
dc.proquest.subject 0479 en_US
dc.proquest.subject 0715 en_US
dc.proquestyes Yes en_US


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