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dc.contributor.supervisor Wieden, Hans-Joachim
dc.contributor.author Vigar, Justin R. J.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2020-01-22T20:51:05Z
dc.date.available 2020-01-22T20:51:05Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5670
dc.description.abstract Translation initiation rates are fine-tuned by altering interactions between the ribosome and the translation initiation region of an mRNA. In bacteria varying levels of RNA structure attenuate these interactions by masking the ribosome-binding site from the small ribosomal subunit. Recent studies have described diverse strategies for recruiting mRNA to the ribosome, and highlighted the contributions of ribosomal protein S1. Here, I provide evidence that the non-canonical initiation mechanism that governs translation of the rpsA mRNA, encoding ribosomal protein S1, contains a three-dimensional architecture that is required for efficient translation. Furthermore, S1 plays an essential role during the initiation phase of translation by recruiting mRNA to the ribosome—unfolding structured mRNAs, and allowing for correct start codon positioning on the ribosome. Combining crosslinking immunoprecipitation and high-throughput sequencing approaches revealed the extent of S1’s involvement in mRNA recruitment, while also highlighting a broader role as a regulator of many RNA classes. en_US
dc.description.sponsorship Alberta Innovates (Strategic Chairs Program) [SC60-T2] en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject RNA en_US
dc.subject Biophysics en_US
dc.subject Synthetic Biology en_US
dc.subject Ribosome en_US
dc.subject Translation Initiation en_US
dc.subject Engineering en_US
dc.subject RNA-seq en_US
dc.subject Dissertations, Academic en_US
dc.title rpsA and ribosomal protein S1: investigating a non-canonical translation initiation element en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0786 en_US
dc.proquest.subject 0537 en_US
dc.proquestyes Yes en_US


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