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dc.contributor.supervisor Wieden, Hans-Joachim
dc.contributor.author Heller, Jalyce L.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2020-01-22T16:46:13Z
dc.date.available 2020-01-22T16:46:13Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5669
dc.description.abstract The rpsA gene contains a translation initiation region (TIR) which lacks a Shine-Dalgarno element, is highly structured, and which efficiently drives translation in bacteria. The rpsA gene is downregulated by its gene product, ribosomal protein S1. To investigate how the structure of the TIR facilitates function both in translation and downregulation, we employed a combined SAXS, SHAPE, and computational approach. The structure-function relationship reveals that the introduction of single nucleotide substitutions in the TIR alters its structure, corresponding to a modulated translation efficiency in vivo. The activity of the rpsA TIR in a minimal in vitro transcription/translation system was investigated to understand the TIR’s requirement for additional factors. We observed that S1 is strictly required for its translation, and that S1 specifically recognizes and binds to the TIR when in excess over ribosomes. The structure-driven mechanism of this TIR may represent a previously overlooked strategy of translation initiation in bacteria. en_US
dc.description.sponsorship Alberta Innovates (Strategic Chairs Program [SC60-T2], Natural Sciences and Engineering Research Council of Canada Graduate Scholarship- Masters en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Ribosomal protein S1 en_US
dc.subject Translation en_US
dc.subject rpsA en_US
dc.subject bacteria en_US
dc.subject translation initiation region en_US
dc.subject Dissertations, Academic en_US
dc.title The rpsA gene and its product ribosomal protein S1: investigating the role of RNA structure in translation en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0786 en_US
dc.proquest.subject 0307 en_US
dc.proquestyes Yes en_US


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