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dc.contributor.supervisor Thakor, Nehal
dc.contributor.supervisor Wieden, Hans-Joachim
dc.contributor.author Balasingam, Nirujah
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2018-07-18T22:06:42Z
dc.date.available 2018-07-18T22:06:42Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/10133/5173
dc.description.abstract hOLA1 is a purine nucleotide binding protein that belongs to the P-loop GTPase family. hOLA1 suppresses protein synthesis by limiting ternary complex formation thus promoting the integrated stress response (ISR). ISR determines cell fate by activating or inhibiting pro- and anti-apoptotic proteins depending on the type of the stress condition. ISR facilitates cell survival in response to mild stress and promotes apoptosis in response to chronic stress. Depletion of hOLA1 leads to increased cell survival and diminished ISR. This study investigates the role of hOLA1 in apoptosis. My hypothesis was that diminished ISR in hOLA1-depleted cells leads to upregulation of anti-apoptotic proteins thus inhibiting apoptosis. Indeed, this study shows that hOLA1 depletion upregulates anti-apoptotic proteins such as cIAP1, cIAP2, and Bcl-xL, thus inhibiting apoptosis. The inhibition of apoptosis was studied by assessing caspase activation, cleaved poly (ADP-ribose) polymerase (PARP) level, and measuring apoptosis by propidium iodide (PI) staining and flow cytometry. en_US
dc.description.sponsorship NSERC ARRTI Innovation Canada en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Adenosine triphosphatase en_US
dc.subject Apoptosis en_US
dc.subject Purine nucleotides en_US
dc.subject Protein binding en_US
dc.subject Breast -- Cancer en_US
dc.subject Genetic translation en_US
dc.subject breast cancer en_US
dc.subject cancer en_US
dc.subject hOLA1 en_US
dc.subject translation en_US
dc.title Elucidating the role of human obg like ATPase1 (hOLA1) in apoptosis en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0379 en_US
dc.proquest.subject 0307 en_US
dc.proquest.subject 0487 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


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