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dc.contributor.author Wang, Ou
dc.contributor.author Liang, Guanxiang
dc.contributor.author McAllister, Tim A.
dc.contributor.author Plastow, Graham
dc.contributor.author Stanford, Kim
dc.contributor.author Selinger, L. Brent
dc.contributor.author Guan, Le Luo
dc.date.accessioned 2018-05-31T20:10:29Z
dc.date.available 2018-05-31T20:10:29Z
dc.date.issued 2016
dc.identifier.citation Want, O., Liang, G., McAllister, T.A., Plastow, G., Stanford, K., Selinger, B., & Guan, L.L. (2016). Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders. PLoS ONE, 11(3), e0151284. doi:10.1371/journal.pone.0151284 en_US
dc.identifier.uri https://hdl.handle.net/10133/5116
dc.description Sherpa Romeo green journal. Open access, distributed under the terms of the Creative Commons Attribution License en_US
dc.description.abstract Super-shedder cattle are a major disseminator of E . coli O157:H7 into the environment, and the terminal rectum has been proposed as the primary E . coli O157:H7 colonization site. This study aimed to identify host factors that are associated with the super-shedding pro- cess by comparing transcriptomic profiles in rectal tissue collected from 5 super-shedder cattle and 4 non-shedder cattle using RNA-Seq. In total, 17,859 ± 354 genes and 399 ± 16 miRNAs were detected, and 11,773 genes were expressed in all animals. Fifty-eight differ- entially expressed (DE) genes (false discovery rate < 0.05) including 11 up-regulated and 47 down-regulated (log 2 (fold change) ranged from -5.5 to 4.2), and 2 up-regulated DE miRNAs (log 2 (fold change) = 2.1 and 2.5, respectively) were identified in super-shedders compared to non-shedders. Functional analysis of DE genes revealed that 31 down-regu- lated genes were potentially associated with reduced innate and adaptive immune functions in super-shedders, including 13 lymphocytes membrane receptors, 3 transcription factors and 5 cytokines, suggesting the decreased key host immune functions in the rectal tissue of super-shedders, including decreased quantity and migration of immune cells such as lym- phocytes, neutrophils and dendritic cells. The up-regulation of bta-miR-29d-3p and the down regulation of its predicted target gene, regulator of G-protein signaling 13 , suggested a potential regulatory role of this miRNA in decreased migration of lymphocytes in super- shedders. Based on these findings, the rectal tissue of super-shedders may inherently exhibit less effective innate and adaptive immune protection. Further study is required to confirm if such effect on host immunity is due to the nature of the host itself or due to actions mediated by E . coli O157:H7. en_US
dc.language.iso en_US en_US
dc.publisher Public LIbrary of Science en_US
dc.subject E-coli 0157:H7 en_US
dc.subject Super-shedder cattle en_US
dc.subject Rectal tissue en_US
dc.subject Immunity en_US
dc.subject Host factors en_US
dc.subject Transcriptomic profiles en_US
dc.title Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Alberta en_US
dc.publisher.institution Lethbridge Research Centre en_US
dc.publisher.institution Alberta Agriculture and Forestry en_US
dc.publisher.institution University of Lethbridge en_US


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