Show simple item record Foroud, Nora A. Shank, Roxanne A. Kiss, Douglas Eudes, François Hazendonk, Paul 2016-12-01T00:30:18Z 2016-12-01T00:30:18Z 2016
dc.identifier.citation Foroud, N. A., Shank, R. A., Kiss, D., Eudes, F., & Hazendonk, P. (2016). Solvent and water mediated structural variations in deoxynivalenol and their potential implicaitons on the disruption of ribosomal function. Frontiers in Microbiology, 7(1239). doi:10.3389/fmicb.2016.01239 en_US
dc.description Sherpa Romeo green journal: open access en_US
dc.description.abstract Fusarium head blight (FHB) is a disease of cereal crops caused by trichothecene producing Fusarium species. Trichothecenes, macrocylicic fungal metabolites composed of three fused rings (A–C) with one epoxidef unctionality, area class of mycotoxins known to inhibit protein synthesis in eukaryotic ribosomes. These toxins accumulate in the kernels of infected plants rendering them unsuitable for human and animal consumption. Among the four classes of trichothecenes (A–D) A and B are associated with FHB, where the type B trichothecene deoxynivalenol (DON) is most relevant. While it is known that these toxins inhibit protein synthesis by disrupting peptidyl transferase activity, the exact mechanism of this inhibition is poorly understood. The three-dimensional structures and H-bonding behavior of DON were evaluated using one-and two-dimensional nuclear magnetic resonance (NMR) spectroscopy techniques. Comparisons of the NMR structure presented here with the recently reported crystal structure of DON bound in the yeast ribosome reveal insights into the possible toxicity mechanism of this compound. The work described herein identifies a waterbinding pocket in the core structure of DON, where the 3OH plays an important role in this interaction. These results provide preliminary insights into how substitution at C3 reduces trichothecene toxicity. Further investigations along these lines will provide opportunities to develop trichothecene remediation strategies based on the disruption of water binding interactions with 3OH. en_US
dc.language.iso en_CA en_US
dc.publisher Frontiers Media en_US
dc.subject Deoxynivalenoi (DON) en_US
dc.subject Fusarium head blight en_US
dc.subject Fusarium graminearum en_US
dc.subject NMR spectroscopy en_US
dc.subject Mycotoxins en_US
dc.subject Chemical structure en_US
dc.title Solvent and water mediated structural variations in deoxynivalenol and their potential implications on the disruption of ribosomal function en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.description.peer-review Yes en_US
dc.publisher.institution Lethbridge Research and Development Centre en_US
dc.publisher.institution University of Lethbridge en_US

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