Show simple item record

dc.contributor.supervisor Golsteyn, Roy
dc.contributor.author Swift, Lucy H
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2015-11-30T18:45:55Z
dc.date.available 2015-11-30T18:45:55Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/10133/3813
dc.description.abstract We investigated checkpoint adaptation in HT-29 colorectal adenocarcinoma cells treated with cisplatin. Cells that undergo checkpoint adaptation arrest at and then abrogate the G2/M checkpoint to enter mitosis with damaged DNA. We identified that cytotoxic amounts of cisplatin induce either checkpoint adaptation or apoptosis in a concentration dependent manner. We also found that some cisplatin treated cells can survive checkpoint adaptation. These survival cells may contain rearranged genomes, because they entered mitosis with damaged DNA. Additionally, cisplatin treated cells can die when they are induced to undergo checkpoint adaptation but entry into mitosis is inhibited. This might prevent cells surviving treatment with rearranged genomes and could improve the efficacy of genotoxic anti-cancer drugs. Finally, we show that the response following checkpoint adaptation is not identical after treatment with two different genotoxic agents; both HT-29 and M059K glioma cells treated with camptothecin spend longer in mitosis than cells treated with cisplatin. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta : University of Lethbridge, Dept. of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject apoptosis en_US
dc.subject checkpoint adaptation en_US
dc.subject cisplatin en_US
dc.subject genotoxic agents en_US
dc.subject mitosis en_US
dc.title Investigation of checkpoint adaptation in human cancer cells treated with the genotoxic agent cisplatin en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Ph.D en_US
dc.proquest.subject 0379 en_US
dc.proquest.subject 0307 en_US
dc.proquest.subject 0992 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record