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dc.contributor.supervisor Thomas, James E.
dc.contributor.supervisor Gannon, Victor P. J.
dc.contributor.author Laing, Chad R.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2014-10-24T19:46:35Z
dc.date.available 2014-10-24T19:46:35Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/10133/3553
dc.description xvi, 206 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract Human infections with Shiga-toxin (Stx)-producing E. coli (STEC) vary in severity of illness. The pan-genome of a bacterial species contains a shared, essential core genome, and a variably distributed accessory genome. While single nucleotide changes likely influence virulence in STEC, horizontal gene transfer (HGT) on elements such as bacteriophage are thought to be most important. My thesis objectives were to: 1) develop tools for the pangenomic analyses of bacterial genomes; 2) describe the phylogeny of STEC and; 3) determine if the evolution of the Stx2-bacteriophage parallels that of its bacterial host. For this thesis, the software program Panseq was created and used to identify pan-genomic differences among STEC. Whole-genome phylogenies showed all serotypes as discrete clusters, with O157:H7 having three distinct lineages and grouping separately from all other STEC. Finally, the phylogenies of Stx2-bacteriophage and their bacterial hosts were largely concordant, with occasional instances of HGT having led to novel pathogen emergence. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Escherichia coli O157:H7 -- Research en_US
dc.subject Bacterial genomes -- Research en_US
dc.subject Bacteriophages -- Research en_US
dc.subject Verocytotoxins -- Research en_US
dc.subject Escherichia coli infections en_US
dc.subject Dissertations, Academic en_US
dc.title The evolution of the pan-genome of Shiga-toxin (Stx) producing Escherichia coli and the Stx₂ bacteriophage en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Ph.D en_US
dc.proquestyes No en_US


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