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dc.contributor.supervisor Abbott, D. Wade
dc.contributor.supervisor Mosimann, Steven C.
dc.contributor.author Farnell, Benjamin J.
dc.date.accessioned 2014-10-14T21:46:18Z
dc.date.available 2014-10-14T21:46:18Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/10133/3540
dc.description.abstract Homogalacturonan (HG) is a structural plant cell wall polysaccharide and a key source of dietary fiber. The human genome does not contain a single enzyme known to be involved in pectin digestion, and therefore, in order to modify HG fibers and potentially extract nutritional value, humans rely on a consortium of symbiotic intestinal bacteria, such as Bacteroides thetaiotaomicron, to deconstruct and to ferment this complex carbohydrate into host absorbable products. B. thetaiotaomicron contains over 300 predicted carbohydrate active enzymes within its genome that are primarily organized into sugar-selective metabolic pathways called Polysaccharide Utilization Loci PULs (PULs). One such PUL (PUL75: BT4108-BT4124), is activated by HG and is believed to contain enzymes that convert polymerized HG into monosaccharides (GalA). This study reports molecular biology, biochemistry, and functional genomics data that characterize the function of PUL75 gene products involved in HG utilization. Based on these findings, a model for the step-wise process of HG recognition, transport and modification by PUL75 is proposed. en_US
dc.description.sponsorship Agriculture and Agri-Food Canada (AAFC) and University of Lethbridge en_US
dc.language.iso en en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Bacteroides thetaiotaomicron en_US
dc.subject Polysaccharide Utilization Loci en_US
dc.subject Pectin en_US
dc.subject Homogalacturonan en_US
dc.subject Utilization Pathway en_US
dc.title Understanding the recognition and utilization of homogalacturonan by Bacteroides thetaiotaomicron en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.degree.level Masters
dc.proquest.subject 0307 en_US
dc.proquest.subject 0487 en_US
dc.proquestyes Yes en_US


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