Show simple item record

dc.contributor.supervisor Wieden-Kothe, Ute
dc.contributor.supervisor Roussel, Marc R.
dc.contributor.author Kamalampeta, Rajashekhar
dc.date.accessioned 2014-07-17T18:21:48Z
dc.date.available 2014-07-17T18:21:48Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/10133/3464
dc.description xiii, 119 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract Pseudouridines (Ψ), the most common modifications in RNA, are formed by stand-alone Ψ synthases in all organisms. In addition, archaea and eukaryotes use H/ACA small ribonucleoproteins for pseudouridylation. Cbf5, the catalytic component of these complexes, can also introduce Ψ55 in archaeal tRNAs in a guide RNA-independent manner. Here, kinetic and thermodynamic analyses revealed that both Pyrococcus furiosus Nop10 and Gar1 proteins enhance the catalytic ability of Cbf5 and increase its affinity for tRNA. Pus10, representing a novel Ψ synthase family, is the in vivo archaeal tRNA Ψ55 synthase. Characterization of several Pus10 variants demonstrated the importance of the thumb loop for catalysis, a potential role of the THUMP domain in tRNA binding and a new catalytic arginine which may flip the target uridine into Pus10’s active site. The quantitative characterization of the archaeal pseudouridine synthases Cbf5 and Pus10 reported here sheds light on their cellular roles in RNA modification. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Pseudouridine -- Research en_US
dc.subject Transfer RNA -- Research en_US
dc.subject Archaebacteria -- Research en_US
dc.subject Dissertations, Academic en_US
dc.title Kinetic analysis of pseudouridine formation in tRNA by archaeal Cbf5 and Pus10 en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Ph.D en_US
dc.degree.level PhD
dc.proquestyes No en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record