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dc.contributor.supervisor Mosimann, Steven C.
dc.contributor.supervisor Selinger, L. Brent
dc.contributor.author Gruninger, Robert J.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2011-07-08T19:43:16Z
dc.date.available 2011-07-08T19:43:16Z
dc.date.issued 2009
dc.identifier.uri https://hdl.handle.net/10133/2473
dc.description xix, 148 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract The structure and mechanism of the Protein Tyrosine Phosphatase-like Phytases (PTPLPs) from Selenomonas ruminantium (PhyAsr) and Mitsuokella multacida (PhyAmm) were investigated using a combination of enzyme kinetics, site-directed mutagenesis, and X-ray crystallography. I show that PTPLPs use a classical protein tyrosine phosphatase catalytic mechanism and adopt a core PTP fold. Several unique structural features of PTPLPs confer specificity for inositol phosphates. The effect of ionic strength and oxidation on the kinetics and structure of PTPLPs was investigated. The structural consequences of reversible and irreversible oxidation on PTPLPs and PTPs are compared and discussed. We determine the structural basis of substrate specificity in PTPLPs and propose a novel reaction mechanism for the hydrolysis of inositol polyphosphates by PTPLPs. Finally, the structure and function of a unique tandemly repeated phytase has been determined. We show that the active sites of the tandem repeat possess significantly different specificities for inositol polyphosphate. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Phytases en_US
dc.subject Protein-tyrosine kinase en_US
dc.subject Phosphorylation en_US
dc.subject Dissertations, Academic en_US
dc.title Structure and mechanism of protein tyrosine phosphatase-like phytases en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Doctor of Philosophy
dc.degree.level PhD
dc.degree.discipline Chemistry & Biochemistry
dc.organization University of Lethbridge
dc.librarysymbol ALU
dc.degree.field Arts and Science
dc.degree.subfield Sciences


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