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dc.contributor.supervisor Kovalchuk, Igor
dc.contributor.author Zhong, Nuanying
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2020-03-20T16:54:42Z
dc.date.available 2020-03-20T16:54:42Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/10133/5697
dc.description.abstract Cannabis sativa is an agriculturally and medicinally important plant with many pharmaceutical properties. Cancer is a deadly disease; it is estimated that it will cause over 80 thousand deaths in 2019 in Canada. Although numerous studies have demonstrated that cannabinoids have anti-tumorous properties in various cancers, the anti-malignant activities of cannabinol (CBN) on carcinogenesis and underlying mechanisms remain largely unknown. In this study, we provide evidence that CBN inhibits proliferation of A172, HB8065 and HCC1806 cells in a dose- and time-dependent manner. CBN regulates expression of cannabinoid receptors, CB2, GPR55 and GPR18 in different cell lines, while reducing levels of phosphorylated ERK1/2 in HCC1806 and phosphorylated AKT in A172 and HB8065 cells. We find that CBN induces apoptosis through downregulation of p21 and p27 and a G1 or S-phase cell cycle arrest through a dose-dependent downregulation of cyclin E1, CDK1 and CDK2. These data support the medicinal potential of CBN in anti-cancer therapy. en_US
dc.description.sponsorship Mitacs en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Cancer cells en_US
dc.subject Cannabinoids en_US
dc.subject Cannabis — Therapeutic use en_US
dc.subject Carcinogenesis en_US
dc.subject Marijuana — Therapeutic use en_US
dc.subject Medical Marijuana — therapeutic use en_US
dc.title Cannabinol inhibits proliferation and induces cell cycle arrest and apoptosis in glioblastoma, hepatocellular carcinoma and breast cancer cells en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquest.subject Cellular biology [0379] en_US
dc.proquest.subject Oncology [0992] en_US
dc.proquest.subject Molecular biology [0307] en_US
dc.proquestyes Yes en_US


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