Show simple item record Zaheer, Rahat Bony-Dugat, Eric Holman, Devon Cousteix, Elodie Xu, Yong Munns, Krysty D. Selinger, Lorna J. Barbieri, Rutn Alexander, Trevor W. McAllister, Tim A. Selinger, L. Brent 2019-08-23T22:55:41Z 2019-08-23T22:55:41Z 2017
dc.identifier.citation Zaheer, R., Dugat-Bony, E., Holman, D., Cousteix, E., Xu, Y., Munns, K.,...Selinger, L. B. (2017). Changes in bacterial community composition of Escherichia coli O157:H7 super-shedder cattle occur in the lower intestine. PLoS ONE, 12(1), e0170050. doi:10.1371/journal.pone.0170050 en_US
dc.description Sherpa Romeo green journal. Open access article. Creative Commons Attribution 4.0 International License (CC BY 4.0) applies. en_US
dc.description.abstract Escherichia coli O157:H7 is a foodborne pathogen that colonizes ruminants. Cattle are considered the primary reservoir of E. coli O157:H7 with super-shedders, defined as individuals excreting>104 E. coli O157:H7 CFU g-1 feces. The mechanisms leading to the super-shedding condition are largely unknown. Here, we used 16S rRNA gene pyrosequencing to examine the composition of the fecal bacterial community in order to investigate changes in the bacterial microbiota at several locations along the digestive tract (from the duodenum to the rectal-anal junction) in 5 steers previously identified as super-shedders and 5 non-shedders. The overall bacterial community structure did not differ by E. coli O157:H7 shedding status; but several differences in the relative abundance of taxa and OTUs were noted between the two groups. The genus Prevotella was most enriched in the non-shedders while the genus Ruminococcus and the Bacteroidetes phylum were notably enriched in the super-shedders. There was greater bacterial diversity and richness in samples collected from the lower- as compared to the upper gastrointestinal tract (GI). The spiral colon was the only GI location that differed in terms of bacterial diversity between super-shedders and non-shedders. These findings reinforced linkages between E. coli O157:H7 colonization in cattle and the nature of the microbial community inhabiting the digestive tract of supershedders. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.subject E. coli en_US
dc.subject Fecal bacterial community en_US
dc.subject Super-shedders en_US
dc.subject Non-shedders en_US
dc.subject Steers en_US
dc.subject.lcsh Escherichia coli O157:H7
dc.subject.lcsh Bacterial diversity
dc.subject.lcsh Beef cattle
dc.title Changes in bacterial community composition of Escherichia coli O157:H7 super-shedder cattle occur in the lower intestine en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution Lethbridge Research and Development Centre en_US
dc.publisher.institution Université Paris-Saclay en_US
dc.publisher.institution University of Lethbridge en_US

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