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dc.contributor.supervisor Kothe, Ute
dc.contributor.author Czekay, Dominic P.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2019-07-29T18:49:21Z
dc.date.available 2019-07-29T18:49:21Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/10133/5488
dc.description.abstract H/ACA guide RNAs are a class of noncoding RNA that direct the pseudouridylation of many cellular RNA species. In most eukaryotes, H/ACA guide RNAs share a conserved hairpin-hinge-hairpin structure, where each hairpin can direct pseudouridylation when associated with evolutionarily conserved core proteins. Target selection occurs by base pairing between target RNA and single-stranded loops within each hairpin of the H/ACA guide RNA, called pseudouridylation pockets. Here, I have analyzed the structure-function relationship of H/ACA guide RNAs by applying a structure-focused approach to design H/ACA guide RNAs for pseudouridylation of novel substrates. Thereby, I designed and tested several artificial H/ACA guide RNAs that were both highly active and specific for their respective substrates in vitro. In addition, I generated multiple sub-optimal H/ACA guide RNA designs that reveal important information regarding H/ACA guide RNA features dictating productivity. My results open new avenues for evaluating, predicting/identifying, and designing cellular guide-substrate RNA combinations. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject artificial guide RNAs en_US
dc.subject cellular guide-substrate RNA combinations en_US
dc.subject H/ACA guide RNA en_US
dc.subject pseudouridylation pocket en_US
dc.subject targeted pseudouridylation en_US
dc.subject Dissertations, Academic en_US
dc.title Structure based H/ACA guide RNA design and testing explains the structure-function relationship of H/ACA guide RNA en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0307 en_US
dc.proquestyes Yes en_US
dc.embargo.start 2019-01-01
dc.embargo.end 2019-07-01
dc.embargo Yes en_US


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