Show simple item record Juranek, Stefan Wieden, Hans-Joachim Lipps, Hans J. 2019-07-18T21:00:12Z 2019-07-18T21:00:12Z 2003
dc.identifier.citation Juranek, S., Wieden, H.-J., & Lipps, H. J. (2003). De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae. Nucleic Acids Research, 31(5), 1387-1391. DOI: 10.1093/nar/gkg233 en_US
dc.description Sherpa Romeo green journal. Permission to archive final published version en_US
dc.description.abstract Dramatic DNA reorganization and elimination processes occur during macronuclear differentiation in ciliates. In this study we analyzed whether cytosine methylation of specific sequences plays a functional role during DNA rearrangement. Three classes of sequences, macronuclear-destined sequences (MDSs, pCE7), members from a large family of transposon-like elements and micronuclear-specific sequences (pLJ01), differing in their structure and future destiny during nuclear differentiation, were studied in the micronucleus, the developing macronucleus and, when present, in the mature macronucleus. While the MDSs become processed to a 1.1 and 1.3 kb gene-sized macronuclear DNA molecule, the family of transposon-like elements represented by MaA81 becomes removed late in the course of polytene chromosome formation. The micronuclear-specific sequence pLJ01 is eliminated together with bulk micronuclear DNA during degradation of polytene chromosomes. No methylated cytosine could be detected in the vegetative macronucleus and no difference in methylation pattern was observed either between micronucleus and developing macronucleus in MDSs or in a micronuclear-speci®c sequence. However, a significant percentage of the cytosines contained in the transposon-like element becomes methylated de novo in the course of macronuclear differentiation. This is the first demonstration that cytosine methylation in speci®c sequences occurs during macronuclear differentiation and may provide a first step towards understanding epigenetic factors involved in DNA processing en_US
dc.language.iso en_US en_US
dc.publisher Oxford University Press en_US
dc.subject Cytosines
dc.subject Cytosine methylation
dc.subject DNA reorganization
dc.subject Macronuclear differentiation
dc.subject.lcsh Cytosine
dc.subject.lcsh Methylation
dc.title De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Witten/Herdecke en_US
dc.publisher.institution University of Lethbridge en_US

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