Show simple item record

dc.contributor.supervisor Thakor, Nehal
dc.contributor.supervisor Roussel, Marc R.
dc.contributor.author Sharma Khandiga, Divya
dc.date.accessioned 2019-06-11T18:45:30Z
dc.date.available 2019-06-11T18:45:30Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/10133/5392
dc.description.abstract Programmed cell death protein 4 (PDCD4) inhibits IRES-mediated translation of anti-apoptotic proteins such as XIAP. PDCD4 was shown to directly interact with the XIAP IRES element and inhibit translation initiation. Additionally, our lab reported that a eukaryotic initiation factor, eIF3 interacts with the XIAP IRES to facilitate ribosome recruitment. Interestingly, the activity of PDCD4 and eIF3 are regulated by common regulatory kinases called S6K1 and 2. Therefore, to investigate the possibility of interaction between PDCD4 and eIF3 as well as their co-regulation by S6K1 and 2, I have performed co-immunoprecipitation assays in glioblastoma and S6K double knockout MEFs. The results of in cellulo assays demonstrate RNA-independent PDCD4-eIF3 interactions. In addition, eIF3F, one of the 13 eIF3 subunits has been demonstrated to interact directly with PDCD4. This study suggests that the interaction of PDCD4 with eIF3F may have a role in regulating global and/or transcript-specific translation. en_US
dc.description.sponsorship SGS en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Genetic translation -- Research en_US
dc.subject Apoptosis -- Research en_US
dc.subject Tumor suppressor proteins -- Research en_US
dc.subject Dissertations, Academic en_US
dc.subject eIF3 en_US
dc.subject PDCD4 en_US
dc.subject PDCD4-eIF3 interaction en_US
dc.subject translation en_US
dc.title Characterizing the interaction between PDCD4 and eIF3 with respect to translation regulation en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0307 en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0379 en_US
dc.proquestyes Yes en_US
dc.embargo.start 2017-01-15
dc.embargo.end 2017-07-15
dc.embargo Yes en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record