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dc.contributor.author Schwindel, C. Daniela
dc.contributor.author Ali, Karim
dc.contributor.author McNaughton, Bruce L.
dc.contributor.author Tatsuno, Masami
dc.date.accessioned 2019-04-23T20:21:48Z
dc.date.available 2019-04-23T20:21:48Z
dc.date.issued 2014
dc.identifier.citation Schwindel, C. D., Ali, K., McNaughton, B. L., & Tatsuno, M. (2014). Long-term recordings improve the detection of weak excitatory-excitatory connections in rat prefrontal cortex. Journal of Neuroscience, 34(16), 5454-5467. DOI:10.1523/JNEUROSCI.4350-13.2014 en_US
dc.identifier.uri https://hdl.handle.net/10133/5334
dc.description Sherpa Romeo yellow journal. Open access article. Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported License (CC BY-NC-SA 3.0) applies. en_US
dc.description.abstract Characterization of synaptic connectivity is essential to understanding neural circuit dynamics. For extracellularly recorded spike trains, indirect evidence for connectivity can be inferred from short-latency peaks in the correlogram between two neurons. Despite their predominance in cortex, however, significant interactions between excitatory neurons (E) have been hard to detect because of their intrinsic weakness. By taking advantage of long duration recordings, up to 25 h, from rat prefrontal cortex, we found that 7.6% of the recorded pyramidal neurons are connected. This corresponds to 70% of the local E–E connection probability that has been reported by paired intracellular recordings(11.6%). This value is significantly higher than previous reports from extracellular recordings, but still a substantial underestimate. Our analysis showed that long recording times and strict significance thresholds are necessary to detect weak connections while avoiding false-positive results, but will likely still leave many excitatory connections undetected. In addition, we found that hyper-reciprocity of connections in prefrontal cortex that was shown previously by paired intracellular recordings was only present in short-distance, but not in long distance (300 micrometers or more) interactions. As hyper-reciprocity is restricted to local clusters, it might be a mini columnar effect. Given the current surge of interest in very high-density neural spike recording (e.g., NIH BRAIN Project) it is of paramount importance that we have statistically reliable methods for estimating connectivity from cross-correlation analysis available. We provide an important step in this direction. en_US
dc.language.iso en_US en_US
dc.publisher Society for Neuroscience en_US
dc.subject Cross correlations en_US
dc.subject Extracellular recordings en_US
dc.subject Local connectivity en_US
dc.subject Medial prefrontal cortex en_US
dc.subject Excitatory neurons
dc.subject.lcsh Prefrontal cortex
dc.subject.lcsh Rats as laboratory animals
dc.title Long-term recordings improve the detection of weak excitatory-excitatory connections in rat prefrontal cortex en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Neuroscience en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Lethbridge en_US
dc.publisher.url https://doi.org/10.1523/JNEUROSCI.4350-13.2014


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