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dc.contributor.author Krishnan, Preethi
dc.contributor.author Ghosh, Sunita
dc.contributor.author Graham, Kathryn
dc.contributor.author Mackey, John R.
dc.contributor.author Kovalchuk, Olga
dc.contributor.author Damaraju, Sambasivarao
dc.date.accessioned 2019-01-23T20:41:31Z
dc.date.available 2019-01-23T20:41:31Z
dc.date.issued 2016
dc.identifier.citation Krishnan, P., Ghosh, S., Graham, K., Mackey, J R., Kovalchuk, O., & Damaraju, S. (2016). Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer. Oncotarget, 7(25), 37944-37956. https://doi.org/10.18632/oncotarget.9272 en_US
dc.identifier.uri https://hdl.handle.net/10133/5272
dc.description Sherpa Romeo blue journal. Open access article. Creative Commons Attribution 3.0 License (CC BY 3.0) applies en_US
dc.description.abstract Piwi-interacting RNAs (piRNAs), whose role in germline maintenance has been established, are now also being classified as post-transcriptional regulators of gene expression in somatic cells. PIWI proteins, central to piRNA biogenesis, have been identified as genetic and epigenetic regulators of gene expression. piRNAs/PIWIs have emerged as potential biomarkers for cancer but their relevance to breast cancer has not been comprehensively studied. piRNAs and mRNAs were profiled from normal and breast tumor tissues using next generation sequencing and Agilent platforms, respectively. Gene targets for differentially expressed piRNAs were identified from mRNA expression dataset. piRNAs and PIWI genes were independently assessed for their prognostic significance (outcomes: Overall Survival, OS and Recurrence Free Survival, RFS). We discovered eight piRNAs as novel independent prognostic markers and their association with OS was confirmed in an external dataset (The Cancer Genome Atlas). Further, PIWIL3 and PIWIL4 genes showed prognostic relevance. 306 gene targets exhibited reciprocal relationship with piRNA expression. Cancer cell pathways such as apoptosis and cell signaling were the key Gene Ontology terms associated with the regulated gene targets. Overall, we have captured the entire cascade of events in a dysregulated piRNA pathway and have identified novel markers for breast cancer prognostication. en_US
dc.language.iso en_US en_US
dc.publisher Impact Journals en_US
dc.subject piRNA en_US
dc.subject PIWI en_US
dc.subject Breast cancer en_US
dc.subject Prognostic marker en_US
dc.subject TCGA en_US
dc.subject Piwi-interacting en_US
dc.subject.lcsh Gene expression
dc.subject.lcsh Piwi genes
dc.subject.lcsh Breast--Cancer
dc.subject.lcsh Breast--Cancer--Research
dc.title Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Alberta en_US
dc.publisher.institution Alberta Health Services en_US
dc.publisher.institution University of Lethbridge en_US
dc.publisher.url https://doi.org/10.18632/oncotarget.9272


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