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dc.contributor.supervisor Eudes, François
dc.contributor.supervisor Hazendonk, Paul
dc.contributor.author Banwo, Inumidun Damilola
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2018-08-07T21:17:39Z
dc.date.available 2018-08-07T21:17:39Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/10133/5180
dc.description.abstract Cell penetrating peptides (CPPs) are short peptide 8-30 amino acids polypeptides that can deliver various molecules (nanoparticles to large fragment DNA) to subcellular locations such as the plastid organelles, cytoplasm, and nucleus etc. CPPs are characterized by containing clusters of cationic side chains that allow them to interact directly with the polar membrane surface, which enables then to enter the cell. One of the function of CPPs are to deliver cargo molecules such as DNA and RNA into cells. Most application have been developed for animal cells; however, the use of CPPs in plant cells remain a small researched field because of insufficient understanding of their mode of uptake. The present research describes firstly, the use of NMR technique to elucidate the structural properties of CPP-DNA complexation. Secondly the dynamic of the complexation. Finally, to relate the observations to their translocation mechanism. For this study, short peptides such as; arginine (R), tri-arginine (R3), nano-arginine (R9), and Tat2 (RKKRRQRRRRKKRRQRRR) were complexed with single-stranded and double-stranded DNA 5’ AGTCC 3’. The interaction between the single-stranded and double-stranded DNA 5’ AGTCC 3’ with the peptides was compared to understand how different DNA strands can influence complex formation. These complexes are used at relatively high concentrations; hence, measurements were at millimolar concentration. Therefore, for the study of DNA-CPP complexes, both non-isotopically enriched DNA and CPP samples were used. The reason for using such samples is to see if complexation can be observed using NMR spectroscopy, which will reduce the use of costly materials. en_US
dc.description.sponsorship University of Lethbridge, Agric and Agricultural Food Canada en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Cells -- Permeability -- Research en_US
dc.subject Plant cell membranes -- Research en_US
dc.subject Nuclear magnetic resonance spectroscopy en_US
dc.subject Biological transport -- Physiology en_US
dc.subject Peptides -- Phsyiological transport en_US
dc.subject cargo molecules en_US
dc.subject cell penetrating peptides en_US
dc.subject cellular uptake en_US
dc.subject NMR spectroscopy en_US
dc.subject plant cells en_US
dc.subject plasma membrane passage en_US
dc.title Nuclear magnetic resonance spectroscopic characterization of peptide based nanocarriers and cargo complexes en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0379 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


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