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dc.contributor.author Krishnan, Preethi
dc.contributor.author Ghosh, Sunita
dc.contributor.author Wang, Bo
dc.contributor.author Heyns, Mieke
dc.contributor.author Li, Dongping
dc.contributor.author Mackey, John R.
dc.contributor.author Kovalchuk, Olga
dc.contributor.author Damaraju, Sambasivarao
dc.date.accessioned 2017-07-13T21:51:17Z
dc.date.available 2017-07-13T21:51:17Z
dc.date.issued 2016
dc.identifier.citation Krishnan, P., Ghosh, S., Wang, B., Heyns, M., Li, D., Mackey, J.R. ...Damaraju, S. (2016). Genome-wide profling of transfer RNAs and their role as novel prognostic markers for breast cancer. Scientific Report, 6, 32843. doi:10.1038/srep32483(2016) en_US
dc.identifier.uri https://hdl.handle.net/10133/4867
dc.description Sherpa Romeo green journal: open access en_US
dc.description.abstract Transfer RNAs (tRNAs, key molecules in protein synthesis) have not been investigated as potential prognostic markers in breast cancer (BC), despite early findings of their dysregulation and diagnostic potential. We aim to comprehensively profile tRNAs from breast tissues and to evaluate their role as prognostic markers (Overall Survival, OS and Recurrence Free Survival, RFS). tRNAs were profiled from 11 normal breast and 104 breast tumor tissues using next generation sequencing. We adopted a Case-control (CC) and Case-Only (CO) association study designs. Risk scores constructed from tRNAs were subjected to univariate and multivariate Cox-proportional hazards regression to investigate their prognostic value. Of the 571 tRNAs profiled, 76 were differentially expressed (DE) and three were significant for OS in the CC approach. We identified an additional 11 tRNAs associated with OS and 14 tRNAs as significant for RFS in the CO approach, indicating that CC alone may not capture all discriminatory tRNAs in prognoses. In both the approaches, the risk scores were significant in the multivariate analysis as independent prognostic factors, and patients belonging to high-risk group were associated with poor prognosis. Our results confirmed global up-regulation of tRNAs in BC and identified tRNAs as potential novel prognostic markers for BC. en_US
dc.language.iso en_US en_US
dc.publisher Nature Publishing Group en_US
dc.subject Transfer RNAs en_US
dc.subject Prognostic marker en_US
dc.subject Overall survival en_US
dc.subject Recurrence free survival en_US
dc.subject Breast cancer en_US
dc.subject Next generation sequencing en_US
dc.title Genome-wide profiling of transfer RNAs and their role as novel prognostic markers for breast cancer en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Alberta en_US
dc.publisher.institution Cross Cancer Institute en_US
dc.publisher.institution University of Lethbridge en_US


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