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dc.contributor.author Ziemienowicz, Alicja
dc.contributor.author Seyed Mohammad, Reza Rahavi
dc.contributor.author Kovalchuk, Igor
dc.date.accessioned 2017-04-28T20:36:55Z
dc.date.available 2017-04-28T20:36:55Z
dc.date.issued 2011
dc.identifier.citation Ziemienowicz, A., Seyed Mohammad, R. R., & Kovalchuk, I. (2011). The stimulatory effect of CaCl2, NaCl and NH4NO3 salts on the ssDNA-binding activity of RecA depends on nucleotide cofactor and buffer pH. BMB Reports, 44(5), 341-346. doi:10.5483/BMBRep.2011.44.5.341 en_US
dc.identifier.uri https://hdl.handle.net/10133/4837
dc.description Open access journal en_US
dc.description.abstract The single-stranded DNA binding activity of the Escherichia coli RecA protein is crucial for homologous recombination to occur. This and other biochemical activities of ssDNA binding proteins may be affected by various factors. In this study, we analyzed the effect of CaCl2, NaCl and NH4NO3 salts in combination with the pH and nucleotide cofactor effect on the ssDNA-binding activity of RecA. The studies revealed that, in addition to the inhibitory effect, these salts exert also a stimulatory effect on RecA. These effects occur only under very strict conditions, and the presence or absence and the type of nucleotide cofactor play here a major role. It was observed that in contrast to ATP, ATPγS prevented the inhibitory effect of NaCl and NH4NO3, even at very high salt concentration. These results indicate that ATPγS most likely stabilizes the structure of RecA required for DNA binding, making it resistant to high salt concentrations. en_US
dc.language.iso en_US en_US
dc.publisher Korean Society for Biochemistry and Molecular Biology en_US
dc.subject DNA-protein interaction en_US
dc.subject Homologous recombination en_US
dc.subject Mono-/divalent ion salts en_US
dc.subject RecA en_US
dc.subject Single-stranded DNA en_US
dc.subject.lcsh DNA-protein interactions
dc.subject.lcsh Protein binding
dc.subject.lcsh DNA
dc.subject.lcsh Salt
dc.subject.lcsh Salts
dc.subject.lcsh Ammonium nitrate
dc.subject.lcsh Calcium chloride
dc.title The stimulatory effect of CaCl2, NaCl and NH4NO3 salts on the ssDNA-binding activity of RecA depends on nucleotide cofactor and buffer pH en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Lethbridge en_US


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