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dc.contributor.author Gomez-Lopez, Nardhy
dc.contributor.author Tanaka, Satomi
dc.contributor.author Zaeem, Zoya
dc.contributor.author Metz, Gerlinde A. S.
dc.contributor.author Olson, David M.
dc.date.accessioned 2017-04-27T18:40:25Z
dc.date.available 2017-04-27T18:40:25Z
dc.date.issued 2013
dc.identifier.citation Gomez-Lopez, N., Tanaka, S., Zaeem, Z., Metz, G.A.S., & Olson, D. M. (2013). Maternal circulating leukocytes display early chemotactic responsiveness during late gestation. BMC Pregnancy & Childbirth, 13(Suppl): 58. Retrieved from http://www.biomedcentral.com/1471-2393/13/S1/S8 en_US
dc.identifier.uri http://hdl.handle.net/10133/4832
dc.description Sherpa Romeo green journal: open access en_US
dc.description.abstract Background: Parturition has been widely described as an immunological response; however, it is unknown how this is triggered. We hypothesized that an early event in parturition is an increased responsiveness of peripheral leukocytes to chemotactic stimuli expressed by reproductive tissues, and this precedes expression of tissue chemotactic activity, uterine activation and the systemic progesterone/estradiol shift. Methods: Tissues and blood were collected from pregnant Long-Evans rats on gestational days (GD) 17, 20 and 22 (term gestation). We employed a validated Boyden chamber assay, flow cytometry, quantitative real timepolymerase chain reaction, and enzyme-linked immunosorbent assays. Results: We found that GD20 maternal peripheral leukocytes migrated more than those from GD17 when these were tested with GD22 uterus and cervix extracts. Leukocytes on GD20 also displayed a significant increase in chemokine (C-C motif) ligand 2 (Ccl2) gene expression and this correlated with an increase in peripheral granulocyte proportions and a decrease in B cell and monocyte proportions. Tissue chemotactic activity and specific chemokines (CCL2, chemokine (C-X-C motif) ligand 1/CXCL1, and CXCL10) were mostly unchanged from GD17 to GD20 and increased only on GD22. CXCL10 peaked on GD20 in cervical tissues. As expected, prostaglandin F2a receptor and oxytocin receptor gene expression increased dramatically between GD20 and 22. Progesterone concentrations fell and estradiol-17b concentrations increased in peripheral serum, cervical and uterine tissue extracts between GD20 and 22. Conclusion: Maternal circulating leukocytes display early chemotactic responsiveness, which leads to their infiltration into the uterus where they may participate in the process of parturition. en_US
dc.language.iso en_US en_US
dc.publisher Biomed Central Ltd en_US
dc.subject Maternal circulating leukocytes en_US
dc.subject Chemotactic en_US
dc.subject Gestation en_US
dc.subject Long-Evans rat en_US
dc.subject Parturtion
dc.subject.lcsh Rats--Pregnancy
dc.subject.lcsh Rats--Parturition
dc.subject.lcsh Leucocytes
dc.subject.lcsh Chemokines
dc.title Maternal circulating leukocytes display early chemotactic responsiveness during late gestation en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Neuroscience en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Alberta en_US
dc.publisher.institution University of Lethbridge en_US


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