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dc.contributor.author Lone, Abdul G.
dc.contributor.author Selinger, L. Brent
dc.contributor.author Uwiera, Richard R. E.
dc.contributor.author Xu, Yong
dc.contributor.author Inglis, G. Douglas
dc.date.accessioned 2017-01-16T21:32:42Z
dc.date.available 2017-01-16T21:32:42Z
dc.date.issued 2013
dc.identifier.citation Lone, A. G., Selinger, L. B., Uwiera, R. R. E., Xu, Y., & Inglis, G. D. (2013). Campylobacter jejuni colonization is associated with a dysbiosis in the cecal microbiota of mice in the absence of prominent inflammation. PLoS ONE 8(9), e75325. doi:10.1371/journal.pone.0075325 en_US
dc.identifier.uri https://hdl.handle.net/10133/4759
dc.description Sherpa Romeo green journal; open access en_US
dc.description.abstract Background: Campylobacter jejuni causes enterocolitis in humans, but does not incite disease in asymptomatic carrier animals. To survive in the intestine, C. jejuni must successfully compete with the microbiota and overcome the host immune defense. Campylobacter jejuni colonization success varies considerably amongst individual mice, and we examined the degree to which the intestinal microbiota was affected in mice (i.e. a model carrier animal) colonized by C. jejuni at high relative to low densities. Methods: Mice were inoculated with C. jejuni or buffer, and pathogen shedding and intestinal colonization were measured. Histopathologic scoring and quantification of mRNA expression for α-defensins, toll-like receptors, and cytokine genes were conducted. Mucosa-associated bacterial communities were characterized by two approaches: multiplexed barcoded pyrosequencing and terminal restriction fragment length polymorphism analysis. Results: Two C. jejuni treatments were established based on the degree of cecal and colonic colonization; C. jejuni Group A animals were colonized at high cell densities, and C. jejuni Group B animals were colonized at lower cell densities. Histological examination of cecal and colonic tissues indicated that C. jejuni did not incite visible pathologic changes. Although there was no significant difference among treatments in expression of mRNA for α-defensins, tolllike receptors, or cytokine genes, a trend for increased expression of toll-like receptors and cytokine genes was observed for C. jejuni Group A. The results of the two methods to characterize bacterial communities indicated that the composition of the cecal microbiota of C. jejuni Group A mice differed significantly from C. jejuni Group B and Control mice. This difference was due to a reduction in load, diversity and richness of bacteria associated with the cecal mucosa of C. jejuni Group A mice. Conclusions: High density colonization by C. jejuni is associated with a dysbiosis in the cecal microbiota independent of prominent inflammation. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.subject Campylobacter jejuni en_US
dc.subject Dysbiosis en_US
dc.subject Cecal microbiota en_US
dc.subject Mice en_US
dc.subject Colonization en_US
dc.subject Mice en_US
dc.title Campylobacter jejuni colonization is associated with a dysbiosis in the cecal microbiota of mice in the absence of prominent inflammation en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution Lethbridge Research Centre en_US
dc.publisher.institution University of Lethbridge en_US
dc.publisher.institution University of Alberta en_US


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